Amplified fragment length polymorphism (AFLP) and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genomic fingerprinting assays were compared for their ability to differentiate Escherichia coli isolates obtained from various host sources, and with respect to their pathogenicity. One hundred and ten verotoxigenic, enterotoxigenic and non-pathogenic E. coli is...

abstract background: regarding the diagnostic errors of the classic criteria including serum ascites albumin gradient (saag), total protein concentration and the adapted light et al’s criteria in distinguishing transudate versus exudates, we evaluated the ascitic fluid to serum bilirubin ratio as a new criteria in this regard. we also evaluated whether the combination of bilirubin ratio with ea...

We investigate the effect of pair creation on a shock structure. Actually, particles accelerated by a shock can be sufficiently energetic to boost, via Inverse Compton (IC) process for example, surrounding soft photons above the rest mass electron energy and thus to trigger the pair creation process. The increase of the associated pair pressure is thus able to disrupt the plasma flow and possib...

Ticks are known or suspected vectors for a wide range of bacterial pathogens. One of the first steps for tick-borne risk assessment is the detection of these pathogens in their vectors. In the present study, a broad-range PCR amplification of the eubacterial gene encoding the 16S rRNA gene combined with Temporal Temperature Gradient gel Electrophoresis (TTGE) was evaluated as a method allowing ...

High risk human papillomaviruses (HR-HPVs) are associated with increased risk of normal cervical cells developing to dysplasia and cervical carcinoma. Therefore, HR-HPV DNA testing can predict an endpoint of cervical carcinogenesis that is earlier than the development of cervical abnormalities. Not only the sensitivity of methods but also the amount of HPV DNA are very important and might be pa...

The current study was conducted to determine the usefulness of 2 molecular techniques, automated repetitive extragenic palindromic-PCR (REP-PCR) and denaturing gradient gel electrophoresis (DGGE), to identify Salmonella serotypes of poultry origin. Salmonella continues to be a foodborne pathogen of principal concern in the United States. The interspersed conserved repetitive sequence of the bac...