Here we combined classical biochemistry with new biophysical approaches to study the organization of glycosylphosphatidylinositol (GPI)-anchored proteins (GPI-APs) with high spatial and temporal resolution at the plasma membrane of polarized epithelial cells. We show that in polarized MDCK cells, after sorting in the Golgi, each GPI-AP reaches the apical surface in homoclusters. Golgi-derived h...

We show that geometric parametric instability (GPI) in graded-index multimode fibers is strongly influenced by higher-order dispersion. By measuring the output spectrum for different core radii, we distinguish peaks generated GPI from other coexisting processes using phase-matching arguments and numerical simulations. highlight first time a non-degenerate process involving two pumps at waveleng...

OBJECTIVES Deep brain stimulation (DBS) is the surgical procedure for patients with advanced Parkinson's disease. Globus pallidus internus (GPi) and subthalamic nucleus (STN) are the most targeted locations for the procedure. To investigate the variable efficiencies for the two different locations, we conducted a meta-analysis to compare both stimulation sites. MATERIALS AND METHODS A systema...

BACKGROUND Mutations in PMM2 impair phosphomannomutase-2 activity and cause the most frequent congenital disorder of glycosylation, PMM2-CDG. Mannose-1-phosphate, that is deficient in this disorder, is also implicated in the biosynthesis of glycosylphosphatidyl inositol (GPI) anchors. OBJECTIVE To evaluate whether GPI-anchor and GPI-anchored proteins are defective in PMM2-CDG patients. METH...

The major glycosylphosphatidylinositols (GPIs) transferred to protein in mammals and trypanosomes contain three mannoses. In Saccharomyces cerevisiae, however, the GPI transferred to protein bears a fourth, alpha1,2-linked Man on the alpha1,2-Man that receives the phosphoethanolamine (EthN-P) moiety through which GPIs become linked to protein. We report that temperature-sensitive smp3 mutants a...

INTRODUCTION Immunization with glucose-6-phosphate isomerase (GPI) induces severe arthritis in DBA/1 mice. The present study was designed to identify the cytokines and co-stimulatory molecules involved in the development of GPI-induced arthritis. METHODS Arthritis was induced in DBA/1 mice with 300 microg human recombinant GPI. CD4+ T cells and antigen-presenting cells from splenocytes of art...

The article by Beier et al1 makes an important contribution to the understanding of paroxysmal nocturnal hemoglobinuria (PNH) bone marrow failure syndrome. We are concerned about application of the statistical technique, which was potentially a consequence of a misconception about the study design. We argue that the unit of analysis should have been cell populations rather than patients. In the...

Receptors involved in the phagocytosis of microorganisms under nonopsonic conditions have been little studied in neutrophils. Complement receptor type 3 (CR3) is a pattern recognition receptor able to internalize zymosan and C3bi-coated particles. We report that Abs directed against CR3 strongly inhibited nonopsonic phagocytosis of Mycobacterium kansasii in human neutrophils. In these cells CR3...

Background: Beta-2 glycoprotein I antibodies (B2-GPI Ab) are significant markers of thrombosis in otoimmune diseases. No literature has been found in Bcr Abl (-) MPNs to exclude the risk of hereditary thrombophilia and to investigate the association of B2-GPI Ab levels with thrombosis. This study aimed to investigate the relation between levels of B2-GPI Ab and thrombosis in BcrAbl (-) MPN with...