T HE ENZYME glutathione peroxidase appears to play a major role in the red cell in the detoxification of potential oxidizing agents. A genetically determined partial, or presumably heterozygous, deficiency of this enzyme has been associated with a transient hemolytic anemia and hyperbilirubinemia in newborns.l* Unless subjected to oxidative stress, red cells from older children and adults with ...

Peroxiredoxin VI (PrxVI) is a bifunctional enzyme with non-selenium glutathione peroxidase and Ca2+-independent acidic phospholipase A2 activities. We demonstrate that transfection-mediated PrxVI overexpression protects immortalized human WI-38 and murine NIH3T3 fibroblasts against cytotoxic doses of tert-butylhydroperoxide and H2O2. Mutants for either glutathione peroxidase or phospholipase A2...

We compared four methods for determination of glutathione peroxidase (EC 1.11.1.9) activity, using blood samples from 52 healthy volunteers. Two methods depended on direct assay of the amount of glutathione remaining at specific time intervals; the two indirect methods involved measuring the rate of disappearance of NADPH. We assessed the precision and reproducibility of each method. One of the...

T HE ENZYME glutathione peroxidase appears to play a major role in the red cell in the detoxification of potential oxidizing agents. A genetically determined partial, or presumably heterozygous, deficiency of this enzyme has been associated with a transient hemolytic anemia and hyperbilirubinemia in newborns.l* Unless subjected to oxidative stress, red cells from older children and adults with ...

The major aim of this work was to investigate how alcohol-induced oxidative stress in combined chemotherapy changes the metabolic function of the liver in experimental animals. This research was conducted to establish how bromocriptine, haloperidol and azithromycin, applied to the experimental model, affected the antioxidative status of the liver. The following parameters were determined: reduc...

This study was conducted to evaluate the hepatoprotective effect of Agaricus blazei Murrill extract (ABM) against experimentally induced carbon tetrachloride (CCl(4)) toxicity in male BALB/c mice. The experiments included a normal group (no induction by CCl(4)), CCl(4-)induction group (with hepatotoxicity by CCl(4) and without treatment) and experimental groups with low dose (200 mg) or high do...

The studies were carried out on 40 young boars of the synthetic line 990. On their 70 days of age, the boars were divided into two groups; the control group received 0.2 mg Se and 30 mg vitamin E while the experimental group received 0.5 mg Se + 60 mg vitamin E per 1 kg of feed mixtures. The feeding test was carried on from 70 days until 180 days of age. During the experiment the boars were sub...