The preservation of cellular ultrastructure by glutaraldehyde is well documented (D. D. Sabatini et al., J. Cell Biol. 17:19, 1963); however, we could find no report of its inactivation of the yellow fever virus in tissue. As laboratory infection of unvaccinated personnel by this virus is also well known (G. P. Berry and S. F. Kitchen, Am. J. Trop. Med. 11:365, 1931), it is important to ascerta...

The deteriorating quality of the environment and consequently stricter emission limits force the produc­ ers of pollutants to modernize their current technolo­ gies, to reduce technologies with toxic materials or to v minimalize emissions. The industrial use of formaldehyde in various fields such as textile, wood, leather, and food process­ ing industry causes a major environmental problem. For...

This study was designed to examine the histologic response of the dental pulp to the use of selected concentrations of glutaraldehyde applied over various time intervals in pulpotomies performed on monkey teeth. One hundred sixty primary teeth from 8 stump-tail macaque monkeys were divided randomly into 10 groups. One group served as the control and received a zinc oxide and eugenol pulpotomy w...

Collagen-based implants have been described as vehicles for transplantation of cultured skin cells for treatment of burn wounds. To optimize vascularization and repair of connective tissue, collagen solubility and glutaraldehyde crosslinking were evaluated. Cultured skin substitutes consisted of human keratinocytes and fibroblasts attached to collagen-glycosaminoglycan substrates that were prep...

The structure of purified preparations of activated (DNA-binding) glucocorticoid receptor (GR) was analyzed in the presence or absence of DNA. A 35-base pair DNA fragment harboring a strong GR-binding site from the mouse mammary tumor virus promoter (-189/-166) was used for stoichiometric analysis of the GR . DNA complex. Glycerol gradient centrifugation was utilized in order to separate the 6 ...

Glutaraldehyde is commonly used in hospitals for cold disinfection of instruments which may be damaged by autoclaving. The increased use of automatic washer/disinfection machines has resulted in a greater risk of spills than with manual methods. A series of experiments was conducted to answer two related research questions: what was the likely range of airborne concentrations when glutaraldehyd...

Orthophthalaldehyde high level disinfectants are contraindicated for use with urological instruments such as cystoscopes due to anaphylaxis-like allergic reactions during surveillance of bladder cancer patients. Allergic reactions and mucosal injuries have also been reported following colonoscopy, laryngoscopy, and transesophageal echocardiography with devices disinfected using orthophthalaldeh...

The aim of this study is to determine suitable conditions for egg disinfection of gilthead sea bream (Sparus aurata), red porgy (Pagrus pagrus), and common dentex (Dentex dentex) with glutaraldehyde, hydrogen peroxide and iodine. The eggs were disinfected with 200 ppm glutaraldehyde (T= 2, 4, 8, 16 min), 300 ppm hydrogen peroxide and iodine (T= 5, 10, 15, 20 min) at four different contact times...

The large increase in passive Na flux that occurs when dog red blood cells are caused to shrink is amiloride sensitive and inhibited when Cl is replaced by nitrate or thiocyanate. Activation and deactivation of this transport pathway by manipulation of cell volume is reversible. Brief treatment of the cells with 0.01-0.03% glutaraldehyde can cause the shrinkage-activated transporter to become i...

SEROTONIN (5-hydroxytryptamine, 5-HT) in blood is transported almost exclusively by circulating platelets.1 Early biochemical and pharmacologic studies demonstrated that most of the 5-HT was found in granules present in platelet hyaloplasm.2” Recently, several laboratories have suggested that platelet serotonin is concentrated within a specific type of platelet organelle, the dense body58 ( Fig...