Chemical and histochemical studies of three lysosomal enzymes-acid phosphatase, ß-glucuronidase, and cathepsinshowed that these enzymes increase during the regression of estrone-induced mammary tumors. One week after removal of the estrone pellet, the tumors had regressed by 50%, at which time acid phosphatase had increased by 67%, cathepsin by 111%, and 0-glucuronidase by 138%. At Week 2, alt...

A cytochemical study of the lysosomal enzyme beta-glucuronidase in 60 cases of acute leukaemia has shown a qualitative difference in the cytoplasmic distribution of the enzyme between blast cells of the lymphoid and myeloid cell series. This difference provides a useful additional method for cytochemical classification of cell type and is superior in this respect to the other lysosomal enzymes ...

The interaction of UICC crocidolite asbestos with biological membranes in vivo was studied in rats after a single intratracheal dose of a suspension of 20 mg of fibres per rat. Development of lung fibrosis (increased level of hydroxyproline, a collagen index together with corresponding pathomorphological alteration) confirmed the penetration of crocidolite fibres into the lungs in the course of...

A histochemical study of hydrolytic enzymes such as acid phosphatase and beta-glucuronidase was made on cultured brain tumor cells. Well-differentiated astrocytic cells, oligodendroglioma cells, and medulloblastoma cells showed low activities. Migrating cells and macrophage-like cells showed intense activities. Meningioma cells and schwannoma cells showed relatively intense activities at the ea...

Abstract A method is proposed for the determination of some ketosteroids in human urine, including enzymatic hydrolysis using β-glucuronidase from E. coli followed by dispersive liquid-liquid microextraction, derivatization analytes with hydroxylamine, and detection reversed-phase ultra-HPLC–quadrupole time-of-flight mass spectrometry. Optimization extraction conditions studied compounds made i...