Graphene quantum dots (GQDs), derived from functionalized graphene precursors are graphene sheets a few nanometers in the lateral dimension having a several-layer thickness. They are zero-dimensional materials with quantum confinement and edge site effects. Intense research interest in GQDs is attributed to their unique physicochemical phenomena arising from the sp²-bonded carbon nanocore surro...

Integrating polypyrrole-cellulose nanocrystal-based composites with glucose oxidase (GOx) as a new sensing regime was investigated. Polypyrrole-cellulose nanocrystal (PPy-CNC)-based composite as a novel immobilization membrane with unique physicochemical properties was found to enhance biosensor performance. Field emission scanning electron microscopy (FESEM) images showed that fibers were nano...

Diabetic therapeutics, including insulin and glucagon-like peptide 1 (GLP-1), are essential for diabetic patients to regulate blood glucose levels. However, conventional treatments that are based on subcutaneous injections are often associated with poor glucose control and a lack of patient compliance. In this review, we focus on the different stimuli-responsive systems to deliver therapeutics ...

A novel needle-type biosensor based on carbon nanotubes is reported. Thebiosensor was prepared by packing a mixture of multi-wall carbon nanotubes (MWCNTs),graphite powder and glucose oxidase (Gox) freeze-dried powder into a glass capillary of 0.5mm inner diameter. The resulting amperometric biosensor was characterizedelectrochemically using amperometry in the presence of hydrogen peroxide and ...

Glucose oxidase (GOx), a flavoenzyme, from Aspergillus niger was produced, purified and immobilized for glucose oxidation. Maximum activities of 0.6 and 0.31U mg fungus dry weight were obtained as intracellular and extracellular respectively. Optimal production (4000 IU/L) was achieved when basal salt medium was supplemented with sucrose (7.5%), peptone (1.5%) phosphorus (0.2%) and MgSO4 (0.2%)...

An implantable glucose microbiosensor that utilizes an electrodeposited redox polymer/glucose oxidase (GOX) sensing membrane and a highly hydrophilic poly (4-vinyl pyridine-co-acrylic acid) (PVP-PAC) overcoating as an analyte-regulating membrane has been developed. A gold microelectrode was electrodeposited with the redox polymer/GOX composite sensing membrane. Two polymers, a hydrophobic poly ...

The presence of pathogenic bacteria in foods has always been a great threat to the wellbeing of people and the revenue of food manufacturers. Therefore, the demand for advanced detection methods that can sensitively and rapidly detect these pathogens has been of great importance. This study reports an electrochemical biosensor for rapid detection of E. coli O157:H7 with the integration of bifun...

The significance of sensing hydrogen peroxide (H2O2) is due to its ubiquity, being a potential biomarker as well an end-product several oxidation reactions. Herein, based on gold nanoparticles (AuNPs) and coupled with single-stranded DNA (ssDNA) ceria (CeO2), we developed novel colorimetric method detect H2O2 glucose in NaCl solutions. In the presence H2O2, ssDNA adsorbed surface CeO2 could be ...

Foundations of biosensor and its technological advancements thereof, was laid down by father of the biosensor, Professor Leland C Clark Jnr., in 1956 with advent of Clark oxygen electrode. The first biosensor dates back to 1962, when Clark and Lyons of the Cincinnati Children’s Hospital proposed the first enzyme based electrode to measure blood glucose[26]. The underlying principle being monito...

A leading biotechnological advancement in the field of biocatalysis is immobilization enzymes on solid supports to create more stable and recyclable systems. Metal-organic frameworks (MOFs) are porous materials that have been explored as for enzyme immobilization. Composed organic linkers inorganic nodes, MOFs feature empty void space with large surface areas ability be modified post-synthesis....