Red blood cell (RBC) velocity and vessel lumen diameter were mea sured in individual microvessels of both normal (mature granulation) and neoplastic (VX2 carcinoma) tissues grown in a transparent ear chamber prior to and following glucose and galactose injections (6 g/kg i.v.) in anesthetized rabbits. RBC velocity was measured using the dual-slit photometric technique and the diameter of the ve...

OBJECTIVE To determine whether infantile colic (IC) is associated with malabsorption of carbohydrates present in fruit juices. METHODS In this double-blind study, parents of 30 healthy infants (5.1 +/- 0.7 months, 7.4 +/- 1.0 kg, 64 +/- 4 cm) were administered a questionnaire to quantitatively assess IC. Thereafter, they were divided into 2 groups, 16 infants with and 14 without IC. Within ea...

Using a quantitative assay for measuring chemotaxis, we tested a variety of sugars and sugar derivatives for their ability to attract Escherichia coli bacteria. The most effective attractants, i.e., those that have thresholds near 10(-5) M or below, are N-acetyl-d-glucosamine, 6-deoxy-d-glucose, d-fructose, d-fucose, 1-d-glycerol-beta-d-galactoside, galactitol, d-galactose, d-glucosamine, d-glu...

Polygalacturonases (PG; EC 3.2.1.15) catalyze the hydrolysis of pectin and/or pectic acid and are useful for industrial applications such as juice clarification and pectin extraction. Growth and heterologous expression of recombinant Saccharomyces cerevisiae which expresses an acidic PG from Aspergillus kawachii has been studied in batch and fed-batch cultures. Kinetics and stoichiometric param...

Melamine (1,3,5-triazine-2,4,6-triamine) is employed in the manufacture of plastics, laminates and glues, yet, it has been found sometimes added illegally to dairy products to artificially inflate foods' protein content. In 2008, dairy products adulterated with melamine were blamed for the death of several infants in China, a situation that forced Beijing to introduce stricter food safety measu...

Yeast growing for a considerable time in glucose 'remember' a previous exposure to galactose, the inducer of its galactose-utilization (GAL) genes. This memory is conveyed by a cytoplasmically transmitted galactokinase working as a signal transducer.

Comparative time-course studies of glycogen synthesis from glucose 6-phosphate, glucose 1-phosphate and UDP-glucose show that glucose 1-phosphate forms glycogen at an initial rate faster than that obtained with glucose 6-phosphate and UDP-glucose. After 5min. the rates from glucose monophosphates are considerably slower. 2,4-Dinitrophenol decreases glycogen synthesis from both glucose monophosp...

Red blood cell (RBC) velocity and vessel lumen diameter were measured in individual microvessels of both normal (mature granulation) and neoplastic (VX2 carcinoma) tissues grown in a transparent ear chamber prior to and following glucose and galactose injections (6 g/kg i.v.) in anesthetized rabbits. RBC velocity was measured using the dual-slit photometric technique and the diameter of the ves...

Galactose oxidase (GOase) was shown to oxidise several C2/C3 fluorinated galactose analogues. Interestingly, the enzyme was able to distinguish between the 2,3-tetrafluorinated galactose and its epimeric glucose analogue, and this represents the first reported biotransformation of a heavily fluorinated sugar.

The kinetics of induced synthesis of glycolytic enzymes of the hybrid yeast Saccharomyces fragilis X Saccharomyces dobzhansizii in response to the addition of glucose or galactose has been studied in a basal medium containing peptone, acetate, and yeast extract. Glucose and galactose bring about a 3to loo-fold increase in specific activity of various glycolytic enzymes during a ‘Thour period. T...