n-acetyl-b-d-glucosaminidase was purified by affinity and ionexchange chromatography. two major, a and b, and three minor intermediate forms were isolated and characterized. nag-a and nag-b were purified 440 and 1200 fold with final yields of 16 and 23 percent respectively. each activity was represented by a single protein band. after 70 min preincubation at 55°c a loss of70% activity of nag-a ...

objective(s): in recent years, there has been an increasing amount of study on early diagnosis of kidney injury through sensitive and specific biomarkers. we examined the practical applicability of the urinary levels of nag (n-acetyl-β-d-glucosaminidase), ap (alkaline phosphatase), and ldh (lactate dehydrogenase) as renal dysfunction screening biomarkers in full and pre-term newborns treated wi...