Plant regeneration was achieved using somatic embryos obtained from the Vitis vinifera cvs Sugraone, Crimson Seedless and Don Mariano. White somatic embryos, 1.5-3 mm in length, were cultured on embryo germination medium with or without IAA (10 μM) + GA3 (1 μM). Germinated embryos showed 5 different morphologies, (1) one cotyledon, (2) two cotyledons, (3) three cotyledons, (4) trumpet-like and ...

Heat-shocking spores at 110 C in 20% sucrose solutions decreased the percentage of the rough variant in a mixed population (rough and smooth variants) of strain M. Heat-shocking spores of the rough variant of strain NCA 1518 in 20% sucrose produced a decline in the number which germinated, whereas the smooth variant of strain NCA 1518 increased in the number which germinated. By the use of phas...