background: in vitro maturation (ivm) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). reliable ivm is an intellectual, scientific and clinical challenge with a number of potential applications. objective: the effect of hcg was evaluated on the timing and regulation of in vitro ovulation for the syrian mice oocytes in the pre...

background: leukemia inhibitory factor (lif) is a 45-56 kda glycoprotein that has an important role in proliferation and embryo implantation. its effect on oocyte maturation and how to exert the function remained to be elucidated. methods: immature mice superovulated with human menopausal gonadotropin and germinal vesicle (gv) oocytes were obtained from ovary 48 hours after. gv oocytes were cul...

Eggs of most animal species are fertilized by a single sperm and fusion of these two cells is essential for the activation of the developmental program of the zygote. Cases in which multiple sperm fertilize an egg, a condition known as polyspermy, the supernumerary paternal centrioles and chromosomes usually create aberrant cell divisions that result in death. Thus, eggs have evolved mechanisms...

background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...