As organisms age, DNA of somatic cells deteriorates, but it is believed that germ cells are protected from DNA-damaging agents. In recent years, this vision has been challenged by studies on humans indicating that genomic instability in germ cells increases with age. However, nothing is known about germ line senescence in wild animals. Here, we examine DNA damage in sperm of a wild vertebrate, ...

Sexual identity is one of the most important factors that determine how an animal will develop. Although it controls many dimorphic tissues in the body, its most ancient role is in the germ line, where it species that some cells become sperm, and others become eggs. In most animals, these two fates occur in distinct sexes. However, certain nematodes like C. elegans produce XX hermaphrodites, wh...

Yeast artificial mini-chromosomes have helped to define the features of chromosome architecture important for accurate segregation and replication and have been used to identify genes important for chromosome stability and as large-fragment cloning vectors. Artificial chromosomes have been developed in human cells but they do not have defined, experimentally predictable structures. Fragments of...

Embryonic patterning and germ-cell specification in mice are regulative and depend on zygotic gene activities. However, there are mouse homologues of Drosophila maternal effect genes, including vasa and tudor, that function in posterior and germ-cell determination. We report here that a targeted mutation in Tudor domain containing 1/mouse tudor repeat 1 (Tdrd1/Mtr-1), a tudor-related gene in mi...

The paper by Johnson et al. "Germ line stem cells and follicular renewal in the postnatal mammalian ovary" published in Nature on the 11th of March [1] suggests that a population of germ line stem cells exist within the adult mouse ovary and that oocyte numbers are regulated by a balance of activation of these cells and cell death. This paper should be viewed in its historical context as it rei...

The origin of the germ cell lineage in vertebrates is a fundamental question that has preoccupied developmental biologists. Recent work on the origin of the avian germ line has extended and clarified our understanding of the temporal and spatial segregation of primordial germ cells (PGC) during prestreak stages of development. The germ cells first appear at Stage X (Eyal-Giladi and Kochav, 1976...

A common feature of development in most vertebrate models is the early segregation of the germ line from the soma. For example, in Xenopus and zebrafish embryos primordial germ cells (PGCs) are specified by germ plasm that is inherited from the egg; in mice, Blimp1 expression in the epiblast mediates the commitment of cells to the germ line. How these disparate mechanisms of PGC specification e...

ZAM is an long terminal repeat (LTR) retrotransposon from Drosophila melanogaster that bears striking resemblance to the vertebrate retroviruses, in their structure and replication cycle. This element transposes via an RNA intermediate and its reverse transcription, and ultimately inserts copies within the germ line. In this paper, we show that intercellular communication established between th...

Background: The objective of this studywas to investigate the effect of high and moderate summer ambient temperatures on testicular structures and endocrine profile of developing ram lambs. Materials and Methods: Twenty fall-born ramlambswere randomly divided into two groups: animalswere kept outdoor (n = 10) under ambient temperature (31-50°C) or maintained indoor (26-32°C) from May to October...

Segregation of the germ line from the soma is an essential event for transmission of genetic information across generations in all sexually reproducing animals. Although some well-studied systems such as Drosophila and Xenopus use maternally inherited germ determinants to specify germ cells, most animals, including mice, appear to utilize zygotic inductive cell signals to specify germ cells dur...