Aloe, an important folk herbal drug, includes abundant polysaccharides and secondary metabolites, which make it difficult to isolate high-quality DNA or RNA. In this paper, one and two improved methods were used to isolate the genomic DNA and RNA from the leaf of aloe, respectively. The obtained samples presented good quality and integrity; thus, they could be further used for many downstream m...

A simple and rapid method is described where human genomic DNA suitable for PCR was prepared from serum by microwave irradiation. We were able to reproducibly amplify single-copy gene sequences up to 442 bp from small quantities of serum without the need for DNA extraction. Genotyping results obtained from serum samples were shown to be identical to those derived from purified DNA from the same...

The aim of this study was to compare the efficiency of DNA extraction from water as well as from blood samples spiked with A. fumigatus spores, using selected commercial kits. Extraction of DNA according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. The efficiency of DNA extraction was measured by PCR using A...

The PrepFiler Forensic DNA Extraction Kit enables isolation of genomic DNA from a variety of biological samples. The kit facilitates reversible binding of DNA with magnetic particles resulting in high DNA recovery from samples with very low and high quantities of biological materials: 0.1 and 40 microL of human blood (donor 2) provided 14 and 2883 ng of DNA, respectively. Following the revised ...

PCR based molecular markers are powerful tools for the analysis of genetic diversity for which isolation of good quality genomic DNA is essential. This paper presents a comparison of three DNA extraction methods for 1, 5 and 10 larvae of Aedes aegypti. First method employing DNAzol® involves simple and one step protocol for DNA isolation where maximum purity (A260/A280 1.9) was obtained with si...

Campylobacter remains the leading cause responsible of human gastroenteritis worldwide. The current study aimed to compare internal DNA extraction protocol with two commercially available kits, using C. jejuni (ATCC® 29428TM) and coli (ATCC®43478TM) validate optimize through artificial contamination confirmation spp. from broiler chickens, turkeys, beef meats samples. processes were carried out...