| MicroRNA (miRNA ) alterations are involved in the initiation and progression of human cancer. The causes of the widespread differential expression of miRNA genes in malignant compared with normal cells can be explained by the location of these genes in cancer-associated genomic regions, by epigenetic mechanisms and by alterations in the miRNA processing machinery. MiRNA-expression profiling o...

Abstract Gene regulatory variations accumulate during evolution and alter gene expression. While the importance of expression variation in phenotypic is well established, molecular basis remains largely unknown. Here, we examine two closely related yeast species, Saccharomyces cerevisiae paradoxus, which show phenotypical differences morphology cell cycle progression when grown same environment...

Contrarily to the traditional view in which only one or a few key genes were supposed to be the causative factors of diseases, we discuss the importance of considering groups of functionally related genes in the study of pathologies characterised by chromosomal copy number alterations. Recent observations have reported the existence of regions in higher eukaryotic chromosomes (including humans)...

BACKGROUND Caffeine enhances the cytocidal effects of DNA-damaging agents. This study investigated genes involved in the synergistic effect of caffeine on osteosarcoma cells using gene-profiling analysis. MATERIALS AND METHODS Sensitivity to cisplatin and the synergistic effect of caffeine were evaluated in five osteosarcoma cell lines with different p53 gene status. Gene expression profiles ...

Circulating tumor cells (CTCs) have potential utility as a surrogate biomarker of tumor biology via a liquid biopsy. The aim of this study was to evaluate if the nCounter NanoString assay could be used for accurate gene expression profiling of CTCs using the PAM50 research-use-only CodeSet. Analysis was performed on CTCs isolated by the ANGLE Parsortix system from healthy blood spiked with the ...

Most RNAs within polarized cells such as neurons are sorted subcellularly in a coordinated manner. Despite advances in the development of methods for profiling polyadenylated RNAs from small amounts of input RNA, techniques for profiling coding and non-coding RNAs simultaneously are not well established. Here, we optimized a transcriptome profiling method based on double-random priming and appl...

Cervical cancer (CC) is the fourth most common cause of death among women globally. Nearly 90% CC mortality occurs in low- and middle-income countries. Hence there an urgent need for identification new biomarkers early diagnosis treatment CC. Current research aims to investigate effect mechanism estrogen receptor 1 aurora kinase A as potential diagnostic cervical cancer. Gene microarray dataset...