Differential display polymerase chain reaction is technically challenging at a number of steps, including reliable reamplification of differentially expressed sequences after they are isolated from an acrylamide gel. As the only source of the sequence found to be of interest is the gel from which it was identified as being differentially expressed, failed attempts at reamplification can be part...

Aloe Vera has gained more attention over the last decade due to its reputable, medicinal, and pharmaceutical and food properties. In present research, firstly the different concentrations of pectin and gum acacia were augmented with aloevera gel and the products so prepared were assessed for texture and stickiness. The selected preparation was then supplemented with orange flavour at different ...

We report the efficient poling of an electro-optic (EO) polymer in a hybrid TiO(2)/electro-optic polymer multilayer waveguide modulator on mesoporous sol-gel silica cladding. The mesoporous sol-gel silica has nanometer-sized pores and a low refractive index of 1.24, which improves mode confinement in the 400-nm-thick EO polymer film in the modulators and prevents optical absorption from the low...

Interferon production was induced in mouse Ehrlich ascites tumor cells by infection with Newcastle disease virus. The interferon produced was purified by precipitation with ammonium sulfate, chromatography on carboxymethyl-Sephadex, treatment with blue dextran and polyethylene glycol, gel filtration on Bio-Gel P-60 and Bio-Gel P-200, chromatography on phosphocellulose, isoelectric focusing, and...

A 57-year-old female presented with facial oedema, cheilitis and stomatitis that started after three overnight applications of the ‘Evolution’TM home tooth-whitening kit. Her symptoms slowly resolved without treatment 3 days after she stopped using the product. The whitening kit includes an individualized plastic dental mould, which is prepared by the dentist, and a whitening gel, which contain...

Aloe vera L. (syn. A. barbadensis Miller.) is a perennial succulent plant belonging to the Liliaceae family. The plant is known traditionally as the “healing plant” and is claimed to possess wound-healing, burn-healing, immunomodulatory, anti-inflammatory, anti-oxidant, antiallergic, anti-diabetic, and UV protective properties. Due to these therapeutic properties A. vera is being used in a vari...

The study aimed to evaluate the in vitro properties of a new gel formulation (P-3086) as anti-aging treatment. Two in vitro methods aimed to assess and compare the efficacy of the gel formulation in reducing oxidative damages, artificially induced by UVA on skin-derived keratinocytes and in promoting the synthesis of collagen, compared with other four formulations. P-3086 reduced reactive oxyge...

Listeriosis is a severe infection with high morbidity and mortality. We report a fatal case of listeriosis in a patient with a history of Crohn's disease who consumed chicken salad purchased from a retail food establishment before developing listeriosis. As part of the regulatory testing programs, the U.S. Department of Agriculture Food Safety and Inspection Service and the Florida Department o...

General procedures for the extraction of Citrus leaf proteins for analysis by two-dimensional electrophoresis (2-DE) were developed through the evaluation and modification of existing methods. Among the methods evaluated, the best results were obtained when Tris-HCl, KCl, and phenol extractions were followed by precipitation with organic solvents to purify and concentrate the samples. The utili...

Conformation changes of the double-stranded DNA helix in response to dehydration were investigated by monitoring, by agarose gel electrophoresis, the linking number of covalently closed circular DNA generated by ligation of linear DNA in the presence of different organic solvents or different temperatures. It was found that: (i) The DNA helix unwinds upon addition of certain organic solvents or...