We describe a new method of extraction and purification of estrogens in low concentration in urine, involving a rapid enzymic hydrolysis of a 10-mL sample, automatic extraction (in a tube) with CHCl3/ethyl acetate, purification by chromatography on a disposable "mini-column" of AG 1-X2, and fluorometry by continuous flow according to Itrich's procedure [Acta Endocrinol. (Copenhagen) 35, 34 (196...

This video documents methods for collecting coastal marine water samples and processing them for various downstream applications including biomass concentration. nucleic acid purification, cell abundance, nutrient and trace gas analyses. For today's demonstration samples were collected from the deck of the HMS John Strickland operating in Saanich Inlet. An A-frame derrick, with a multi-purpose ...

Low background miniaturized proportional counters as developed for the GALLEX solar neutrino experiment can be applied to the detection of radioactive noble gas nuclides at very low activities. We have developed an apparatus that allows the activity of trace amounts of isotopes of the four noble gases Ar, Kr, Xe and Rn to be measured. The technique includes contamination-free chromatographic pu...

......................................................................................................................................... 1 Chapter 1: Introduction ............................................................................................................... 2 1.1. Background ..........................................................................................................

This issue of the RPC workshop occurs in a particular historical moment which is characterized by a number of RPC systems already in data taking or in phase of advanced commissioning. In particular the startup of the Cern LHC, foreseen this year, will be a crucial test for the trigger and the time-of-flight RPC systems that have been developed for both trigger and time-of-flight by most of the ...

The work is devoted to the development of an algorithm for selection dust and gas purification equipment in order reduce anthropogenic impact on environment from emissions heat, power chemical industries, which are sources complex environmental pollution. need research was necessitated by inefficiency measures taken due huge number devices with different designs emissions’ purification, their n...

Benzyl Alcohol Dehydrogenase (BADH) is an important enzyme for hydrocarbon degradation, which can oxidize benzyl alcohols to aldehydes, while being capable of catalyzing a reversible reaction by reducing benzaldehyde. BADH is a member of medium chain alcohol dehydrogenases, in which zinc and NAD are essential for enzyme activity. This paper describes the expression, purification, and characteri...