Different levels of beta-galactosidase are found in various trp-lac fusion strains. These levels of beta-galactosidase fall within a 60-fold range. The amount of thiogalactoside transacetylase activity detected in these same strains only varies 10-fold and is found in amounts greater than those predicted from the beta-galactosidase levels. The observation that the beta-galactosidase and thiogal...

Abstract This study investigated the mechanism of lactose assimilation in Nannochloropsis oceanica for dairy-wastewater bioremediation and co-production valuable feed/food ingredients a circular dairy system (β-galactosidase omega-3 polyunsaturated fatty acids). Mixotrophic cultivation was found to be mandatory N. , with biomass production mixotrophic cultures reaching fourfold increase over th...

We previously demonstrated that rats treated with repeated, intermittent amphetamine displayed enhanced amphetamine-mediated dopamine release in the striatum. In this study, we examined whether amphetamine pretreatment would elicit enhanced amphetamine-mediated dopamine release in a cultured cell line in the absence of intact synaptic connections. PC12 cells pretreated with 1 microM amphetamine...

Gene therapy approaches have been suggested for the treatment of cardiovascular disease. Recently, direct transfer of the gene encoding beta-galactosidase into peripheral arteries of the pig has been demonstrated. To determine whether this approach is applicable to other arterial beds and to other species, we first evaluated the use of beta-galactosidase as a marker protein in the canine model....

Recombinant retrovirus vectors are widely used for gene transfer studies. The recent development of a pseudotyped Moloney murine leukemia virus vector that contains the G envelope protein from the vesicular stomatitis virus allows for efficient concentration of vector and offers hope for potential use of these vectors for gene expression in vivo. A standard amphotropic vector expressing a serum...

INTRODUCTION The lacZ gene is frequently fused to heterologous promoters to reveal the expression of endogenous genes in transgenic mice (Bonnerot and Nicolas, 1993; Goring et al., 1987) and in the detection of genetic recombination events in cell fate mapping experiments (Joyner and Zervas, 2006; Petit et al., 2005; Watson et al., 2008). The most popular substrate for the detection of galacto...

In Klebsiella aerogenes P-galactosidase and histidase are subject to strong catabolite repression by glucose in the presence of a good source of nitrogen. Under conditions of nitrogen limitation, the catabolite repression of histidase is relieved, while fi-galactosidase remains strongly repressed. Exogenous adenosine 3’, 5’-monophosphate relieves the catabolite repression of both histidase and ...

Successful therapy for many inherited disorders could be improved if the intervention were initiated early. This is especially true for lysosomal storage disorders. Earlier intervention may allow metabolic correction to occur before lipid buildup has irreversible consequences and/or before the immune system mounts limiting responses. We have been developing gene therapy to treat lysosomal stora...

The release kinetics of beta-galactosidase protein have been determined during small-scale ultrasonication of E. coli cells. Among several studied parameters, ionic strength and cell concentration have the least influence on the rate of protein recovery, whereas sample volume and acoustic power dramatically affect the final yield of soluble protein in the cell-free fraction. The analysis of the...