نتایج جستجو برای: g cloning

تعداد نتایج: 503379  

Journal: :Frontiers of Physics in China 2021

In conventional quantum mechanics, no-deleting and no-cloning theorems indicate that two different nonorthogonal states cannot be perfectly deterministically deleted cloned, respectively. Here, we investigate the deleting cloning in a pseudo-unitary system. We first present pseudo-Hermitian Hamiltonian with real eigenvalues two-qubit By using operators generated from this Hamiltonian, show it i...

Journal: :Yuridika 2023

"Reproductive" cloning and "therapeutic" or "research" are both deliberate attempts to create humans that genetically identical. Human reproductive in general is prohibited by a number of international regional agreements, including the Charter Fukushima, Additional Protocol Council Europe Convention on Rights Biomedicine, World Health Organization resolutions implications for human health, Uni...

Journal: :FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1997
J Wess

G-protein-coupled receptors (GPCRs) play fundamental roles in regulating the activity of virtually every body cell. Upon binding of extracellular ligands, GPCRs interact with a specific subset of heterotrimeric G-proteins that can then, in their activated forms, inhibit or activate various effector enzymes and/or ion channels. Molecular cloning studies have shown that GPCRs form one of the larg...

Ghasemzadegan, H., Rezaei, N., Shahabi, M., Sharifi, Z.,

Abstract Background and Objectives In Iran, Khorasan province is an endemic area for HTLV-1 virus. Considering the inability of serological tests to determine HTLV-1 in window period, their failure to confirm the indetermination results of western blot, and given the probability for HTLV-1 transfusion transmission, a SYBR green-based Real Time PCR was set to measure the HTLV-1 proviral load.  ...

Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...

Journal: :Methods 2012
Kangkan Halder Martin Benzler Jörg S Hartig

DNA and RNA G-quadruplexes have gained increasing attention due to their potential role in a wide range of biological functions. The majority of functional studies characterize the influence of quadruplexes in gene expression including transcription and translation. Many of these studies have used reporter assays to elucidate the effect of quadruplexes at certain positions in promoters and untr...

Journal: :Cancer research 1972
B A Chabner P L Chello J R Bertino

We have studied the antitumor activity of a folate-cleaving bacterial enzyme, carboxypeptidà se G, (CPD G,), which hydrolyzes the C-terminal glutamate residue from folie acid analogs. CPD GÌcompletely inhibited the growth of all murine leukemic cell lines tested in tissue culture at enzyme levels of 0.02 to 0.025 unit/ml. A decrease in cell number was apparent after 12 hr of exposure to the en...

Journal: :The Journal of biological chemistry 1987
B Akerström E Nielsen L Björck

Protein G, the immunoglobin G-binding surface protein of group C and G streptococci, also binds serum albumin. The albumin-binding site on protein G is distinct from the immunoglobulin G-binding site. By mild acid hydrolysis of the papain-liberated protein G fragment (35 kDa), a 28-kDa fragment was produced which retained full immunoglobulin G-binding activity (determined by Scatchard plotting)...

Journal: :International journal of systematic bacteriology 1997
J Kausar Y Ohyama H Terato H Ide O Yamamoto

The nearly complete sequence of the 16S rRNA gene of an extremely highly radiotolerant bacterium, Rubrobacter radiotolerans (reclassified from Arthrobacter radiotolerans based on chemical characteristics), was determined by PCR amplification of the genomic DNA followed by cloning of the amplified gene and sequencing by the dideoxynucleotide method. The sequence was aligned with the sequences of...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1984
R J Legerski D B Brown C A Peterson D L Robberson

An assay has been developed in which excision repair deficiency of xeroderma pigmentosum cells is transiently complemented, as measured by unscheduled DNA synthesis, by microinjection of cytoplasmic poly(A)+ RNA derived from HeLa cells. Four different complementation groups of xeroderma pigmentosum have been assayed. Groups A and G showed complementation, whereas groups D and F did not. Surviva...

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