The present study was to improve the success for the cryopreservation of stallion spermatozoa, especially as the costs of using low post-thaw motility or sub-optimal fertility deters owners and breeders from using frozen semen. The aim was to develop a freezing extender/process that supports cell survivability throughout the freezing. Eleven stallions were collected and frozen in INRA 96 with t...

Methods were developed and evaluated for the preservation of tissue cells grown in suspension culture and the reestablishment of suspension cultures directly from inoculum stored at -175 C. The factors investigated were processing pH, temperature of processing, freezing medium, and method of inoculation of the starter suspension cultures from the frozen stock (-175 C). Three parameters, cell vi...

– In the framework of schematic hard spheres lattice models for granular media we investigate the phenomenon of the " jamming transition ". In particular, using Edwards' approach, by analytical calculations at a mean field level, we derive the system phase diagram and show that " jamming " corresponds to a phase transition from a " fluid " to a " glassy " phase, observed when crystallization is...

This study was conducted to evaluate the activities of antioxidant enzyme (glutathione peroxidase (GSH-Px)) and lysosomal enzymes (alpha-glucopyranosidase (AGP) and beta-N-acetyl-glucosaminidase (BNAG)) of the longissimus dorsi (LD) muscle from Hanwoo (Korean cattle) in three freezing conditions. Following freezing at -20, -60, and -196℃ (liquid nitrogen), LD samples (48 h post-slaughter) were ...

Freezing defined as the complete absence of body movements is a normal response of animals to unavoidable fear stimuli. The present review presents a series of evidence relating different defensive patterns with specific anxiety disorders. There are at least four different kinds of freezing with specific neural substrates. The immobility induced by stimulation of the ventral column of the peria...

St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] is the least cold-hardy turfgrass species. Development of freezing-tolerant St. Augustinegrass cultivars would greatly benefit home owners in many southern states of the US. Towards this breeding goal, 7800 plants regenerated through tissue culture and 36 germplasm accessions were screened for improved freezing tolerance. Among the con...

The relation between long-term decrements of the acoustic startle response in rats and the development of freezing behavior during habituation training was examined. Freezing behavior developed over the initial trials of habituation training, and the rate of long-term response decrements was found to be inversely related to the development of freezing. Manipulations (neurological or behavioral)...

low temperature is one of main factors limiting distribution of woody plants. in order to evaluate the resistance of 4-years old seedlings and adult trees of maclura pomifera to freezing stress, a factorial experiment was carried out based on completely randomized design with three replications in the faculty of agriculture, university of tehran. during 2012-2013 sampling were done from the ann...

The fact that freezing caused marked changes in oxidative activity of tissues was of comparatively little interest until it became apparent that most if not all the enzymatic activities of cells could be reproduced under suitable conditions in cell-free systems. Isolated soluble enzymes were usually unaffect,ed or even stabilized by freezing, and damaging effects of freezing on other enzymes co...