Blood group A + H substance purified from hog gastric mucin was fixed to polystyrene particles by physical absorption to produce a lectin-agglutinable latex. Agglutination of this latex by soybean agglutinin, concanavalin A, and wheat germ agglutinin is specifically inhibited by the respective monosaccharide haptens and is not affected by known noninhibitory sugars. The sensitivity of the assay...

Spherical electron-dense organelles in the perinuclear region of cultured guinea pig aortic smooth muscle cells were identified as lysosomes by their ability to accumulate acridine orange and by cytochemical demonstration of their acid phosphatase content. The number and size of lysosomes increased in subcultured cells. The elemental composition of the lysosomes was quantitated by electron prob...

The aim of this study was to determine the effect incorporation apple puree and maltodextrin agar sol on sorption properties structure dried gel. different drying methods behaviour aerated gels also observed. with addition 25% 40% concentration or without were prepared dried. foamed gel subjected freeze-drying, air-drying vacuum-drying. (adsorption isotherms, water uptake in time) investigated....

Despite the high potential of freeze-casting technique for production porous inorganic substrates, there is a lack studies on tubular geometries and their mechanical behavior under different pressure scenarios. In this work, freeze-cast alumina substrates was assessed by mathematical models from experimental O-ring tests. The stress distributions revealed concentration tensile stresses (within ...

Probiotics are microorganisms that when ingested in high enough numbers beneficially affect the host’s health. To reach these beneficial results, it is necessary to maintain good viability of the probiotic strains during processing, storage and consumption. Stress conditions that usually affect viability of probiotics during production are the following: large concentration of fermentation by-p...

Capillary electrophoresis with laser-induced fluorescence detection was used to monitor gene expression in individual mammalian cells using the reverse transcriptasepolymerase chain reaction. Specifically, beta-actin expression in single LNCaP (prostate cancer) cells was measured. A sieving matrix containing hydroxypropyl methyl cellulose was used to effect size-based separation. Ethidium bromi...

This paper analyzes the determinants of secondary debt market liquidity, identifying conditions under which trading in competitive markets results in sufficient ownership concentration to induce ex post efficient debt relief. The feasibility of debt relief is path-dependent, hinging upon interim economic conditions. Secondary debt markets are likely to freeze during recessions, precisely when t...

Cholesterol was assayed on the Greiner GSA II and the Technicon SMA 12/60 and SMA II autoanalyzers, with Allain's entirely enzymic method (Allain et al. (1974), Clin. Chem. 20, 470--475). The coefficients of variation in the results over a period of one month were less than 6% with the GSA II and the SMA II, but up to 9% on the SMA 12/60. Although the accuracy of the enzymic method depends on t...