After cyanogen bromide digestion of human serum albumin, three fragments, A, B, and C, are obtained. Two different types of a.fEnity columns of Sepharose with L-tryptophan were prepared, one with a free carboxyl group and one with a free amino group. Only Fragment C showed affinity to the Sepharose column with the carboxyl group of tryptophan free, while the column having the amino group free d...

After cyanogen bromide digestion of human serum albumin, three fragments, A, B, and C, are obtained. Two different types of a.fEnity columns of Sepharose with L-tryptophan were prepared, one with a free carboxyl group and one with a free amino group. Only Fragment C showed affinity to the Sepharose column with the carboxyl group of tryptophan free, while the column having the amino group free d...

In the title compound, C24H20N6O, the pyridin-3-yl groups on the ethyl-ene fragment are found in a trans conformation with a C(py)-C(e)-C(e)-C(py) (py = pyridine, e = ethylene) torsion angle of 179.2 (3)°. The dihedral angle between the pyridine rings is 3.5 (1)°. In the crystal, N-H⋯N and C-H⋯O=C inter-actions form a layer arrangement parallel to the bc plane. The compound displays disorder of...

The photoionization and fragmentation of octadecane were investigated with infrared laser desorption/tunable synchrotron vacuum ultraviolet (VUV) photoionization mass spectrometry (IRLD/VUV PIMS) and theoretical calculations. Mass spectra of octadecane were measured at various photon energies. The fragment ions were gradually detected with the increase of photon energy. The main fragment ions w...

Comparisons of recently published primary sequences of staphylococcal and streptococcal pyrogenic toxins prompted an evaluation of biological and immunological properties of the C terminus of staphylococcal enterotoxin C1. The 59 N-terminal amino acids were deleted from the toxin by digestion with trypsin. The resulting fragment (Mr, 20,659) contained the remaining 180 C-terminal residues. This...

Five structural genes coding for the reaction center (RC) L, M, and H subunits and the two light-harvesting (LH) I polypeptides, B870alpha and B870beta, have been mapped on two restriction fragments from the R-prime plasmid pRPS404. It has been recently shown that enhanced near-infrared fluorescence mutants of Rhodopseudomonas capsulata typically lack RC or LH I polypeptides and that these lesi...

Structure elucidation of biological compounds is still a major bottleneck of untargeted LC-HRMS approaches in metabolomics research. The aim of the present study was to combine stable isotope labeling and tandem mass spectrometry for the automated interpretation of the elemental composition of fragment ions and thereby facilitate the structural characterization of metabolites. The software tool...

A 0.8-kilobase SacI DNA fragment in the distal 5'-noncoding region of the c-Ha-ras1 oncogene hybridized to high guanine X cytosine sites of herpes simplex virus type 1 (HSV-1) DNA restriction fragments. Nucleotide sequence comparisons localized one of these sites to the intergenic region of HSV between the immediate-early genes coding for IEmRNA-3 and IEmRNA-4/5 that has enhancer-type activity....

Polypeptide 3, the major membrane-penetrating protein of the human erythrocyte membrane, was characterized, together with two major fragments derived by specific proteolysis of the native protein in the membrane. One fragment (fragment 3f) was obtained from thermolysin cleavage in the extracellular region of the protein, and the other (fragment T1) was derived from tryptic cleavage in the intra...

An enantioselective synthesis of the C(33)-C(37) tripropionate fragment of Amphotericin B has been developed in only 6 steps.