background: in vitro maturation (ivm) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). reliable ivm is an intellectual, scientific and clinical challenge with a number of potential applications. objective: the effect of hcg was evaluated on the timing and regulation of in vitro ovulation for the syrian mice oocytes in the pre...

Objective In the present study, the effects of alginate (ALG) concentration and ovarian cells (OCs) on the development and function of follicles were simultaneously evaluated. MaterialsAndMethods In the first step, preantral follicles (100-130 µm in diameter) were mechanically isolated from the ovaries of 2-week-old NMRI mice, encapsulated in the absence or presence of OCs in 0.5, 0.75 and 1% A...

Although hair follicle stem cells are central to the homeostasis and regeneration of adult follicles, their developmental process had not been fully elucidated. Here, by combining marker-independent long-term three-dimensional (3D) live imaging single-cell transcriptomics, we provided dynamic four-dimensional (4D) atlas with cellular dynamics, cell lineages, transcriptome changes in entire epit...

BACKGROUND Previous studies have demonstrated that pre-pubertal aryl hydrocarbon receptor knockout (AHRKO) mice have slow antral follicle growth and reduced capacity to produce estradiol compared to wild-type (WT) mice. Although previous studies have suggested that this is likely due to a reduced ability of the AHRKO follicles to respond to follicle-stimulating hormone (FSH), this possibility w...

BACKGROUND We have previously shown that suspension culture prevents follicle flattening and maintains three-dimensional follicle architecture better than culture on flat plates. However, many of the follicles cultured in suspension do eventually rupture, as basement membrane integrity is lost and the three-dimensional structure of the follicle is altered. Therefore, the objective of this study...

OBJECTIVE Ovarian and follicle transplantation may preserve fertility in young cancer survivors. In this study, we have transplanted preantral follicles using fibrin gel as a carrier and fibrin gel supplemented with platelet lysate (PL) as a rich source of angiogenic and growth factors. The purpose of this study was to evaluate the role of fibrin gel and PL in follicle transplantation. MATERI...

BACKGROUND The influence of multifollicular growth on pregnancy rates in subfertile couples undergoing intrauterine insemination (IUI) with controlled ovarian hyperstimulation (COH) remained unclear. METHODS Relevant papers were identified by searching MEDLINE, EMBASE and the Cochrane Library. A meta-analysis was performed and Mantel-Haenszel pooled odd ratios (ORs) and risk differences with ...

The extent, causes, and physiological significance of the variation in number of follicles growing during ovarian follicular waves in human beings and cattle are unknown. Therefore, the present study examined the variability and repeatability in numbers of follicles 3 mm or greater in diameter during the follicular waves in bovine estrous cycles, and we determined if the variation in number of ...

A laboratory colony of Culiseta melanura (Coquillett) was used to follow ovarian development from emergence to oviposition and to validate the accuracy of using follicular dilatations to age grade females. We observed no change in the size of the primary follicles in unfed females from emergence to 3 d of age. Sugar feeding stimulated follicular growth and produced the following 3 types of ovar...

In vitro ovarian follicle cultures may provide fertility-preserving options to women facing premature infertility due to cancer therapies. An encapsulated three-dimensional (3-D) culture system utilizing biomaterials to maintain cell-cell communication and support follicle development to produce a mature oocyte has been developed for the mouse. We tested whether this encapsulated 3-D system wou...