We report the development of a multiple-trap laser tweezers Raman spectroscopy (LTRS) array for simultaneously acquiring Raman spectra of individual cells in physiological environments. This LTRS-array technique was also combined with phase contrast and fluorescence microscopy, allowing measurement of Raman spectra, refractility, and fluorescence images of individual cells with a temporal resol...

The effect of chemical composition on the Raman spectra of a series of natural calcalkaline silicate glasses has been quantified by performing electron microprobe analyses and obtaining Raman spectra on glassy filaments (~450 µm) derived from a magma mingling experiment. The results provide a robust compositionally-dependent database for the Raman spectra of natural silicate glasses along the c...

The Stepanov equation, relating the intensity of emission, f(e)(v), at a given frequency, and that of absorption, k(v), at the same frequency, is applied, in its modified form (see equation 3 in text) to suspensions of Chlorella, Porphyridium, and Anacystis and to chlorophyll solutions. This application can reveal whether the yield of fluorescence, Phi(v), is constant, or changes with frequency...

We demonstrate the ability to discriminate between five brands of commercially available extra-virgin olive oil (EVOO) using Raman spectroscopy or fluorescence spectroscopy. Data was taken on both a 'bulk optics' free space system and on a compact handheld device, each capable of taking both Raman and fluorescence data. With the compact Raman device we achieved an average sensitivity and specif...

Cinnamaldehyde is an important compound of the cinnamon essential oil, and it is responsible for the most of the health benefits of cinnamon. Enriching foods such as milk with cinnamaldehyde can lead to greater utilization of cinnamaldehyde properties. In this study, we investigated the interaction of cinnamaldehyde with bovine alpha-lactalbumin. Analyzing the spectrum of alpha-lactalbumin in t...

Eosin is one of the original dyes of xanthenes derivatives. It is also one of the main derivatives of fluorescein and has brilliant fluorescence. Eosin disodium salt called "Eosin Y" is the most useful form of this compound. In common procedures, eosin is usually obtained by the action of bromine on fluorescein, which has not good yield and purity degree. In this research, it is prepared by the...

Multiphoton micro-spectroscopy, employing diffraction optics and electron-multiplying CCD (EMCCD) cameras, is a suitable method for determining protein complex stoichiometry, quaternary structure, and spatial distribution in living cells using Förster resonance energy transfer (FRET) imaging. The method provides highly resolved spectra of molecules or molecular complexes at each image pixel, an...

The binding properties on alizarin to human serum albumin (HSA) have been studied for the first time using fluorescence spectroscopy in combination with UV-visible absorbance spectroscopy. The results showed that alizarin strongly quenched the intrinsic fluorescence of HSA through a static quenching procedure, and non-radiation energy transfer occurred within the molecules. The number of bindin...

Neuronal networks in vitro are prominent systems to study the development of connections in living neuronal networks and the interplay between connectivity, activity and function. These cultured networks show a rich spontaneous activity that evolves concurrently with the connectivity of the underlying network. In this work we monitor the development of neuronal cultures, and record their activi...