Heavy metal and metalloid poisoning in the environment food has piqued public’s interest since it poses significant hazards to ecological system human health. In food, several metals, including cadmium (Cd), lead (Pb), mercury (Hg), tin (Sn), manganese (Mn), aluminium (Al), metalloids, arsenic (As), antimony (Sb), selenium (Se), pose a severe threat It is of utmost importance detect even minute...

Matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometry has become a promising alternative for high-throughput drug discovery as new instruments offer high speed, flexibility and sensitivity, and the ability to measure physiological substrates label free. Here we developed and applied high-throughput MALDI TOF mass spectrometry to identify inhibitors of the salt...

Regulatory monitoring for most antibiotic residues in edible poultry tissues is often accomplished with accurate, although expensive and technically demanding, chemical analytical techniques. The purpose of this study is to determine if a simple, inexpensive bioassay could detect fluoroquinolone (FQ) residues in chicken muscle above the FDA established tolerance (300 ppb) comparable to a liquid...

This paper reviews the following problems, sampling, decomposition procedures and most important analytical methods used for selenium determination, e.g., neutron activation analysis, atomic absorption spectrometry, gas-liquid chromatography, spectrophotometry, fluorimetry, and x-ray fluorescence. This review covers the literature mainly from 1975 to 1977.

METHODS. Recombinant human C-crystallin was mixed with various concentrations of glutathione (GSH) and diamide at 25°C for 1 hour. The extent of glutathiolation of the Ccrystallin was determined by mass spectrometry. Native and S-glutathiolated C-crystallins were labeled with I, and proteolytic degradation was determined using both lens fiber lysate and reticulocyte lysate as sources of ubiquit...

The measurement of the aflatoxin B(1)-lysine serum albumin adduct in human blood samples is the most facile biomarker for the assessment of chronic exposure to aflatoxin B(1). Many technologies have been developed for the measurement of this protein adduct including immunoassays, high-performance liquid chromatography (HPLC) with fluorescence detection, and a newly developed isotope-dilution ma...

Methods Reduced ovomucoid in raw eggwhite was detected using a fluorescence-labeled alkylation probe. The common antioxidants glutathione and cystein were used for in vitro linearization and effects were monitored by CD-spectrometry. Egg-allergic patients were tested serologically (n=19) and by skin prick test (n=9) for IgE against native and linearized ovomucoid and optionally its cooked state.

An aqueous solution was successfully prepared using a low-molecular-weight chitosan oligomer and FITC, and its structural and fluorescent properties were observed by using ¹H NMR, 13C NMR, FT-IR, XRD, UV-Vis, and PL spectrometry. Its application as a metal ion sensor was also evaluated. The fluorescence in the water-soluble chitosan oligomer was a result of the carbamato anion (NHCOO-), and a s...

The performance of fluorescence correlation spectrometry (FCS) was examined for studying the solutions suspended with the fluorescent particles of various sizes from 50 nm to 10 microm in diameter and for different sizes of pinholes: the particles were made to move by simply stirring the solution in the quartz fluorescence cuvette. Without using any magnification tool for the optical image, thi...