Stomata regulate gas exchange through the aerial plant epidermis by controlling the width of a pore bordered by two guard cells. Little is known about the genes that regulate stomatal development. We screened cotyledons from ethyl methanesulfonate-mutagenized seeds of Arabidopsis by light microscopy to identify mutants with altered stomatal morphology. Two mutants, designated too many mouths (t...

Herein, we extend our "combined electrochemical-frustrated Lewis pair" approach to include Pt electrode surfaces for the first time. We found that the voltammetric response of an electrochemical-frustrated Lewis pair (FLP) system involving the B(C6 F5 )3 /[HB(C6 F5 )3 ]- redox couple exhibits a strong surface electrocatalytic effect at Pt electrodes. Using a combination of kinetic competition s...

This is a preliminary study in which we use a genetic algorithm (GA) to breed normalized postfix expressions for solving the facility layout problem (FLP). Our technique, initially developed for VLSI floorplanning, simultaneously places rectangles onto a planar site and optimizes area utilization by altering the shapes of facilities that have fixed area but flexible height and width dimensions....

EBNA-LP-associated proteins were identified by sequencing proteins that immunoprecipitated with Flag epitope-tagged EBNA-LP (FLP) from lymphoblasts in which FLP was stably expressed. The association of EBNA-LP with Hsp70 (72/73) was confirmed, and sequences of DNA-PK catalytic subunit (DNA-PKcs), HA95, Hsp27, prolyl 4-hydroxylase alpha-1 subunit, alpha-tubulin, and beta-tubulin were identified....

Despite the rapid development of frustrated Lewis pair (FLP) chemistry over the last ten years, its application in catalytic hydrogenations remains dependent on a narrow family of structurally similar early main-group Lewis acids (LAs), inevitably placing limitations on reactivity, sensitivity and substrate scope. Herein we describe the FLP-mediated H2 activation and catalytic hydrogenation act...

'Abstract'Sperm DNA was isolated from vaginal fluid and/or endocervical mucoid plugs at various postcoital intervals. Samples were obtained from 25 volunteer couples. DNA was also prepared from the blood and semen of the couples. The DNA was amplified by polymerase chain reaction (PCR) with DlS8O primers. The Fragment length polymorphism of the amplified products (AMP-FLP) was analysed by acryl...

The Flp-FRT site-specific recombination system from Saccharomyces cerevisiae is a powerful and efficient tool for high-throughput genetic analysis of bacteria in the postgenomic era. This review highlights the features of the Flp-FRT system, describes current bacterial genetic methods incorporating this technology and, finally, suggests potential future uses of this system. In combination with ...

The fuzzy primal simplex method [15] and the fuzzy dual simplex method [17] have been proposed to solve a kind of fuzzy linear programming (FLP) problems involving symmetric trapezoidal fuzzy numbers. The fuzzy simplex method starts with a primal fuzzy basic feasible solution (FBFS) for FLP problem and moves to an optimal basis by walking truth sequence of exception of the optimal basis obtaine...

Linear programming (LP) is the most widely used optimization technique for solving real-life problems because of its simplicity and efficiency. Although conventional LP models require precise data, managers and decision makers dealing with real-world optimization problems often do not have access to exact values. Fuzzy sets have been used in the fuzzy LP (FLP) problems to deal with the imprecis...

A transgene was inserted at several different chromosomal sites in Drosophila melanogaster, where its expression was subject to genomic position effects. Quantitative position effects and variegated and constant patterned position effects were observed. We investigated the status of the affected gene in the somatic cells where it normally functions. The FLP site-specific recombinase was used to...