Investigations have been made to develop an efficient protocol for identification and quantification of phenolic acids and flavonoids in hairy roots (HR) of Hypericum perforatum L. HR were induced from root segments of in vitro grown seedlings from H. perforatum, after co-cultivation with Agrobacterium rhizogenes A4. Transgenic status of HR was confirmed by PCR analysis using rolB specific prim...

In South America, the mesocarp flour of Prosopis species plays a prominent role as a food resource in arid areas. The aim of this work was the characterization of the phenolic antioxidants occurring in the pod mesocarp flour of Chilean Prosopis. Samples were collected in the Copiapo, Huasco and Elqui valleys from the north of Chile. The samples of P. chilensis flour exhibited a total phenolic c...

An UHPLC-PDA-ESI/HRMS(n) profiling method was used to identify the glucosinolates and flavonoids of Rorippa indica (Cruciferae), a wild vegetable and Chinese herb used to treat cough, diarrhea, and rheumatoid arthritis. Thirty-three glucosinolates, more than 40 flavonol glycosides, and 18 other phenolic and common organic compounds were identified. The glucosinolates and polyphenols were separa...

Clitoria ternatea L. (CT) (Family: Fabaceae) commonly known as 'Butterfly pea', a traditional Ayurvedic medicine, has been used for centuries as a memory enhancer, nootropic, antistress, anxiolytic, antidepressant, anticonvulsant, tranquilizing and sedative agent. A wide range of secondary metabolites including triterpenoids, flavonol glycosides, anthocyanins and steroids has been isolated from...

Members of several genera of Asteraceae, belonging to the tribes Mutisieae, Cardueae, Lactuceae (all subfamily Cichorioideae), and of Astereae, Senecioneae, Helenieae and Heliantheae (all subfamily Asteroideae) have been analyzed for chemodiversity of their exudate flavonoid profiles. The majority of structures found were flavones and flavonols, sometimes with 6- and/or 8-substitution, and with...

Leaves of a novel strain of peas (Pisum sativum L.) were used to determine the distribution of secondary metabolites and their biosynthetic enzymes. Leaf epidermal layers in this strain are easily separated from the parenchyma. Anthocyanins and flavonol glycosides were localized in epidermal vacuoles only. Among the biosynthetic enzymes studied, phenylalanine ammonia-lyase (PAL, EC 4.3.1.5), S-...

The Brönsted acidic ionic-liquid [HO(3)S(CH(2))(4)mim] HSO(4), a novel dual catalyst-solvent, has been successfully applied in simultaneous microwave-assisted extraction and hydrolysis for the determination of flavonol glycosides in Ginkgo foliage. The parameters, namely the [HO(3)S(CH(2))(4)mim]HSO(4) concentration, microwave-irradiation power, microwave-irradiation time, and solid-liquid rati...

Emanuel L. Johnson3 * and Walter F. Schmidtb a USDA ARS Weed Science Laboratory Bldg 001 Rm. 329 BARC-W Beltsville, MD 20705-2350 USA. Telefax: (301) 504-6491. E-mail: [email protected] b USDA Environmental Chemistry Laboratory, Bldg 12 Beltsville, MD 20705-2350 USA * Author for correspondence and reprint requests Z. Naturforsch. 54c, 881-888 (1999); received April 12/May 18, 1999 Eryth...

Formulations of tomato puree with grape skin fibres (Chardonnay variety) having varying particle sizes were studied. The contents of flavonoids (by HPLC-DAD) and proanthocyanidins (n-butanol/HCl assay), reducing capacity (ferric ion reducing antioxidant power, FRAP) and anti-glycation activity by a bovine serum albumin (BSA)/fructose model system were analysed in vitro. A liking test was perfor...

Ginkgo biloba is among the most sold medicinal plants of this world with estimates of sales in the USA of more than $249 million (de Kosky et al., 2008). Most of the sales are of special extracts from the leaves and are mainly used for the improvement of peripheral and central blood circulation (DeFeudis, 1998). Important constituents present in the leaves are terpene trilactones, i.e., ginkgol...