To gain a better understanding of the interactions among bacteria, viruses and flagellates in coastal marine ecosystems, we investigated the effect of viral lysis and protistan bacterivory on bacterial abundance, production and diversity [determined by 16S rRNA gene polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE)] in three coastal marine sites with different n...

A facultatively filamentous bacterium was isolated from eutrophic lake water and was identified as Flectobacillus sp. strain MWH38 (a member of the Cytophaga-Flavobacterium-Bacteroides phylum) by comparative 16S rRNA gene sequence analysis. Filament formation by Flectobacillus sp. strain MWH38 and filament formation by Flectobacillus major, the closest known relative of strain MWH38, were studi...

A small free-living freshwater bacteriotrophic flagellate Neobodo borokensis n. sp. was investigated by electron microscopy and analysis of its SSU ribosomal RNA gene. This protist has paraxonemal rods of typical bodonid structure in the flagella, mastigonemes on the proximal part of the posterior flagellum, two nearly parallel basal bodies, a compact kinetoplast, and discoid mitochondrial cris...

The 3-D coupled biophysical model ECOSMO (ECOSystem MOdel) has been applied to simulate the spatial and temporal variability of primary and secondary production and biomass in the North Sea in 1984. In order to assess the spatial and temporal dynamics of these components, statistical methods based on empirical orthogonal function analysis (EOF) are introduced to biological oceanography and ecos...

The amoeba-to-flagellate transformation of Naegleria gruberi was studied, exploring the role of calcium in control of this pattern of morphogenesis. A direct and an indirect role for calcium are postulated based on experimental results. Direct inhibition in the presence of calcium is caused by ionophore A23187, substances with ionophore-like activity (amphotericin) and the hormone calcitonin, w...

The fine structure of the mature spermatozoon of the insect Thermobia domestica has been investigated. This flagellate spermatozoon is unique with respect to the relative positions of the centriole, nucleus, mitochondrial nebenkern derivatives, and acrosome along the length of the cell. The acrosome lies at the posterior end of the nucleus. Unlike spermatozoa of most animals, the Thermobia sper...

Tetramitus exhibits independent ameboid and flagellate stages of remarkable morphological dichotomy. Transformation of the ameba involves the formation of four kinetosomes and their flagella. The arrangement of these kinetosomes and associated whorls of microtubules extending under the pellicle establishes the asymmetric flagellate form. While no recognizable kinetosomal precursors have been se...

A fluorimetric microassay that uses a redox dye to determine the viability of the flagellate Trichomonas vaginalis has been optimised to provide a more sensitive method to evaluate potential trichomonacidal compounds. Resazurin has been used in recent years to test drugs against different parasites, including trichomonadid protozoa; however, the reproducibility of these resazurin-based methods ...

The microtubule associated system I fibers of the basal apparatus of the flagellate green alga Spermatozopsis similis are noncontractile and display a 28-nm periodicity. Paracrystals with similar periodicities are formed in vitro by SF-assemblin, which is the major protein component of system I fibers. We have determined the amino acid sequence of SF-assemblin and show that it contains two stru...