There are more than 80 million infertile couples around the world (1). It is estimated that about 40 million of infertile couples are currently seeking infertility treatments (2). Treatment of infertility ranges from simple advice on natural conception to complex and expensive interventions including Assisted Reproductive Technologies (ART) (3). Advanced infertility treatment methods are costly...

The present study was carried out to examine the effect of maturation media on male pronucleus formation of pig oocyte matured and fertilized in vitro. Follicular oocytes collected from prepubertal gilts at a local slaughter house were cultured (36 h) in three different media (mTCM-199, Waymouth MB 752/l, and mTLP-PVA), fertilized in vitro, and assessed for nuclear maturation and male pronucleu...

BACKGROUND It has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. These methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. OBJECTIVE This study compared simple density gradient methods and a combin...

The only known means of effectively separating populations of X and Y bearing sperms is the Beltsville sexing technology. The technology implies that each individual sperm is interrogated for DNA content, measuring the intensity of the fluorescence after staining the spermatozoa with Hoechst 33342. Because there are no data regarding the effect of the staining on stallion sperm, ejaculates were...

In veterinary practice, buoyancy disorders are common in goldfish, often having a sudden onset and present floating at the surface or lying on the bottom. Fifty eight affected fish were examined in detail over a period of 11.5 years, of which 47 were goldfish: there were almost equal numbers of short-bodied and long-bodied goldfish. In many cases, there were no external lesions and no consisten...

Background: Herbal plants often contain active pharmacological compounds they are commonly used for the treatment and prevention of different diseases. Objectives The present study was aimed to identify the effects of different concentrationsof Erucasativa extraction (ESE) on human sperm motility for infertile male in vitro. Methods: Thirty five semen samples were obtained from men (mean age 27...

PURPOSE The aim of this work was to evaluate nuclear histone acetylation level and total histone acetyltransferase (HAT) and deacetylase (HDAC) activity in ejaculated sperm and their relevance to conventional sperm parameters. MATERIALS AND METHODS Thirty-three normozoospermic men were included in this study. Semen samples were processed by swim-up and then immunostained by six acetylation an...

BACKGROUND Leukocytes represent the predominant source of reactive oxygen species both in seminal plasma and in sperm suspensions and have been demonstrated to negatively influence sperm function and fertilization rate in assisted reproduction procedures. Peroxidase test is the standard method recommended by WHO to detect semen leukocytes but it may be inaccurate. The aims of this study were (i...

Major aneuploidies diagnosed prenatally involve the autosomes 13, 18, and 21, and sex chromosomes. Fluorescence in situ hybridization (FISH) allows rapid analysis of chromosome copy number in interphase cells. The purpose of this study was to evaluate the role of multicolor fluorescence in situ hybridization in simultaneous detection of probe sets for chromosome 18, X, and Y in uncultured amnio...