Objective: To explore a fast and sensitive method for screening T cell receptor (TCR) biases through comparison of silver staining for polyacrylamide gel electrophoresis and ethidium bromide staining for agarose gel electrophoresis. Methods: The lymphocytes were isolated from peripheral blood which collected from ten patients with T1DM; the total RNA was extracted followed by transformed to cDN...

Two-dimensional electrophoresis is a major separating technique for proteins in proteomics. Alignment of gel images is critical for intra-laboratory or even more difficult inter-laboratory gel comparisons. In the paper, we propose a novel iterative closest point (ICP) method for 2D-gel electrophoresis image alignment. The paper seeks to introduce an information theoretic measure as one part of ...

We have been developing an image analysis system named DDGEL for 2D gel electrophoresis of genomic DNA. Recently, we have developed a program module for nding a correspondence of spots between two gel electrophoresis images.

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, buffers were used widely to separate any DNA molecule. In this paper, four types previously known compare which is easy for preparation, simple in structure, low cost good performance agarose gel electrophoresis. For this, two concentration (1%, 2%) ladder (10...

Forensic DNA analysis has vastly evolved since the first forensic samples were evaluated by restriction fragment length polymorphism (RFLP). Methodologies advanced from gel electrophoresis techniques to capillary and now next generation sequencing (NGS). Capillary was still is standard method used in analysis. However, dependent upon information needed, there are several different that can be t...

Medicinal plants have been used in traditional medicine for a long time. These contain phytochemicals that variety of medicinal properties. However, accurate identification and authentication plant species ensured their safety efficacy. DNA barcoding using molecular markers has proven to be useful method identification. The rbcL marker was detailed characterization, amplification, phylogenetic ...