Removing sericin from the periphery of silk without damage to fibroin (SF) obtain high-molecular-weight SF is a major challenge in field SF-based biomaterials. In this study, four neutral proteases, subtilisin, trypsin, bromelain and papain, were used degum silk, degumming efficiency proteases their influence on molecular weight (MW) regenerated studied. The results indicated that all could rem...

Achieving a high number of inter-pore channels and a nanofibrous structure similar to that of the extracellular matrix remains a challenge in the preparation of Bombyx mori silk fibroin (BSF) scaffolds for tissue engineering. In this study, Antheraea pernyi silk fibroin (ASF) micro/nano fibers with an average diameter of 324 nm were fabricated by electrospinning from an 8 wt % ASF solution in h...

The titanium and its alloys are used as orthopedic dental implants due to their mechanical and bio-inert properties. The bare metal implants are not the ultimate answer for better osteogenesis and implant integration. Physical and chemical modifications are carried out to achieve the goal of improved adhesion and differentiation of the osteoblast. In this work, the silk fibroins from both mulbe...

Films of regenerated silk fibroin (RSF) are usually brittle and weak, which prevents its wide application as a structural material. To improve the mechanical properties of RSF film, uniaxial extension under swollen conditions was employed to introduce preferred orientation of molecular chains of silk fibroin. Such a prestretching treatment resulted in the strain at break, ultimate stress, Young...

Platinum(iv) complexes take advantage of the exclusive conditions that occur within the tumor to carry out their cytotoxic activity. On the other hand, silk fibroin has natural properties which make it very interesting as a biomaterial: high biocompatibility, biodegradability, low immunogenicity, high cellular penetration capacity and high reactive surface. Herein we report the preparation of s...

Chitosan biopolymer represents an attractive alternative to other bio materials because of its significant physico– chemical behaviours. In order to improve chitosan’s performance, chemical modification has been carried out. Chemical modification that lead to the formation of chitosan derivatives, grafted chitosan and chitosan composites have gained much attention, extensively studied and widel...

In order to use a simple and ecofriendly method to prepare porous silk scaffolds, aqueous silk fibroin solution (ASF) was extracted from silk gland of 7-day-old fifth instar larvae of Bombyx mori (B. mori). SDS-page analysis indicated that the obtained fibroin had a molecular weight higher than 200 kDa. The fabrication of porous scaffolds from ASF was achieved by using the freeze-drying method....

A novel design of silk-based scaffold is developed using a custom-made winding machine, with fiber alignment resembling the anatomical criss-cross lamellar fibrous orientation features of the annulus fibrosus of the intervertebral disc. Crosslinking of silk fibroin fibers with chondroitin sulphate (CS) was introduced to impart superior biological functionality. The scaffolds, with or without CS...

The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomaterials. Silk fibroin (SF), extracted mainly from silkworms, has become a popular biomaterial due to its excellent biocompatibility, exceptional mechanical pro...

Components of silk including silk fibroin have long been used as anti-diabetic remedies in oriental medicine. However, detailed mechanisms underlying these antidiabetic effects remain unclear. In this study, we examined the anti-diabetic activity of silk fibroin hydrolysate (SFH) in C57BL/KsJ db/db (db/db) mice, a well-known animal model of non-insulin dependent diabetes mellitus. When the db/d...