We have established 3 new human colorectal cancer cell lines (LS41 IN, LS513, and I .SI 034) from clinical biopsy samples. These lines are tumorigenic and grow s.c. as adenocarcinomas in nude mouse xenografts. Specific marker chromosomes are observed in each line. Carcinoembryonic antigen is expressed at the surface of all 3 lines, but with marked quantitative differences. Indeed, less than 10%...

Using T-lymphocyte and granulocyte colony assays with truly proliferating cells the effects of the polyamine spermine and of other naturally-occurring inhibitors of cell proliferation have been differentiated. It has been confirmed that spermine, in the presence of fetal calf serum, is a potent inhibitor of cell proliferation. This inhibition could be reversed by the addition of either 3-hydrox...

BACKGROUND Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigat...

ZR-75-1 human mammary cancer cells can be grown in serumfree medium in an estradiciand tamoxifen-sensitive manner. Growth occurs both in monolayer and suspension culture, al though serum is necessary during the initial plating of the cells in monolayer culture. Optimal stimulation of proliferation by estradiol occurs at 10~8 and 10~9 M. Tamoxifen alone weakly stimulated growth in a dose-depende...

ZR-75-1 human mammary cancer cells can be grown in serumfree medium in an estradiciand tamoxifen-sensitive manner. Growth occurs both in monolayer and suspension culture, al though serum is necessary during the initial plating of the cells in monolayer culture. Optimal stimulation of proliferation by estradiol occurs at 10~8 and 10~9 M. Tamoxifen alone weakly stimulated growth in a dose-depende...