NAADP is a highly potent mobilizer of Ca(2+), which in turn triggers Ca(2+)-induced Ca(2+) release pathways in a wide range of species. Nevertheless, NAADP is not presently classified as a second messenger because it has not been shown to increase in response to a physiological stimulus. We now report a dramatic increase in NAADP during sea urchin egg fertilization that was largely due to produ...

Background: This study was carried out to assess the effects of MnTBAP, a cell permeable antioxidant, on motility, membrane integrity, capacitation status and in vitro fertilization ability of frozen-thawed ram semen. Materials and Methods: Fresh semen ejaculates were collected with artificial vagina from five rams, mixed and divided into five equal fractions, and diluted (1:20 v/v) with commer...

The requisite endpoint of mammalian oocyte maturation, whether in vivo or in vitro, is a metaphase II oocyte which is able to be fertilized and which can eventually support normal embryonic development. Oocytes which have been matured in vivo basically fulfill these criteria. On the other hand, a completely different situation exists when these cells are isolated from the ovaries and cultured i...

Sperm nuclear morphometry quantifies properties of the nucleus and allows comparison between semen samples. Chromatin packaging is one of the properties reflected by nuclear measures, and has been related to fertility as correct packaging is essential for not only fertilization but also successful early embryonic development. One such morphometric measure is texture. Nuclear texture is thought ...

The ferruginous limpet is one of the most threatened invertebrate species in Mediterranean Sea. Its controlled reproduction has been considered potentially effective strategies for production juveniles population restocking or recolonization areas where was brought to extinction by human pressure but now under protection (e.g., MPAs). This approach pursued at least two decades with partial resu...

IN the analysis of events immediately preceding and following fertilization of the frog egg, it is frequently desirable to treat the eggs with various reagents. Any such treatment must circumvent the barrier represented by the fertilization membrane and the jelly layers. These membranes can be removed from fertilized eggs mechanically or by treatment with proteolytic enzymes (Spiegel, 1951) wit...

Fertilizer efficiency directly affects the production of continuous cropping greenhouse cucumber. So analyzing the fertilizer efficiency is of great significance in providing decision-making support for fertilization of greenhouse cucumber. By establishing the DEA models of continuous cropping greenhouse cucumber and taking existing data samples as study objects, the problems of fertilizer effi...

Birth of babies through in vitro fertilization procedures has Tenewed interest by clinicians as well as laymen in the phenomenon of fertilizatien. In most medical text books, fertilization is treated very lightly giving students the impressien that fertilization is a simple mechanical penetration of a spermatozoon into an egg. This is far frorn the truth. Fertilization comprises ・a series of co...

This work was designed to study how this ability is affected by different sperm treatments routinely used for in vitro fertilization (IVF) assay. In this study, boar sperm samples from epididymal or ejaculated origin were processed by three different methods: left unwashed (NW group), washed in Dulbecco's phosphate-buffered saline supplemented with 0.1% BSA (BSA group), and washed on a Percoll(...