Beet molasses successfully replaced glucose as sole carbon source to produce poly-b-hydroxybutyrate by a recombinant Escherichia coli strain (HMS174/pTZ18u-PHB). The fermentation with molasses was cheaper than with glucose. The final dry cell weight, PHB content and PHB productivity were 39.5 g/L, 80% (w/w) and 1 g/Lh, respectively, in a 5 L stirred tank fermenter after 31.5 h fed-batch ferment...

In this paper Neuro-dynamic programming (NDP) is proposed as an alternative to alleviate the “curse of dimensionality” of the Dynamic programming (DP) for optimal control of a fed-batch fermentation process in the L-lysine production. The most effective and cheapest method for the Llysine biosynthesis (in biological active form) is the microbiological method via a direct fermentation. In this p...

The main objective of the presented work is to compare two model predictive control strategies by applying them to an antibiotic production in a fed-batch fermentation process. The reactor is modelled as a nonlinear biological compartment system with 13 states. The well-known nonlinear model predictive control (NMPC) is compared with a control strategy based on online optimization of the full t...

Escherichia coli produces insulin, interferons, growth factors, exoand endoproducts, e.g. enzymes, etc. In this paper we present a new approach for multicriteria decision making – InterCriteria Analysis to mathematical modelling of a fermentation process. It is based on the apparatus of index matrices and intuitionistic fuzzy sets. The approach for multicriteria analysis makes it possible to co...

A segregated model was proposed to investigate the inherent relationships between growth, substrate consumption, cell differentiation and product formation in a Bacillus subtilis fermentation producing heterologous amylase in a 22-l bioreactor. The segregated model includes three distinguishable cell states and the transition from vegetative phase to sporangium and finally to mature spore. An a...