AbstrAct The last decade has seen development of numerous new microbial source tracking (MST) methodologies, but many of these have been tested in just a few laboratories with a limited number of fecal samples. This method evaluation study examined the specificity and sensitivity of 41 MST methodologies by analyzing data generated in 27 laboratories. MST methodologies targeting human, cow, rumi...

Uropathogenic Escherichia coli (UPEC) strains are responsible for the majority of uncomplicated urinary tract infections, which can present clinically as cystitis or pyelonephritis. UPEC strain CFT073, isolated from the blood of a patient with acute pyelonephritis, was most cytotoxic and most virulent in mice among our strain collection. Based on the genome sequence of CFT073, microarrays were ...

Waterborne diseases represent a significant public health risk worldwide and can originate from contact with water contaminated with human fecal material. We describe a real-time quantitative PCR (qPCR) method that targets a genetic marker of the human-associated Bacteroides dorei for identification of human fecal pollution in ambient water samples. The following protocol includes water sample ...

Cardiovascular and renal hemodynamic function, nitrogen, electrolyte, and water balances, and circulating eosinophils have been studied before and during the formation of aseites produced by thoracic inferior caval constriction. The data show a striking decrease in the renal and fecal excretion of sodium (Na) and an elevation in fecal potassium (K) output. An analysis of possible factors which ...

Infant fecal samples are commonly studied to investigate the impacts of breastfeeding on the development of the microbiota and subsequent health effects. Comparisons of infants living in different geographic regions and environmental contexts are needed to aid our understanding of evolutionarily-selected milk adaptations. However, the preservation of fecal samples from individuals in remote loc...

conclusions with regard to improving the understanding of the differences in the biodiversity of the fecal microbiota between rd infants and h infants, the findings of this study can provide a possible basis to reveal the relationship between rd and intestinal microbiota. objectives this study aimed to reveal the changes in the biodiversity of the fecal microbiota of infants with rd. patients a...

background: the objective of this study was to evaluate the efficacy of sanitization of lettuce according to the protocols set forth by iranian ministry of health and medical education for reducing populations of total coliform, fecal coliform, and helminth eggs present on lettuce. methods: in the present study, we determined the load of total coliform, fecal coliform, and parasites of lettuce....

hemorrhoidectomy is the treatment of choice for patients with third or fourth-degree hemorrhoids. although the majority of surgeons believe that surgical hemorrhoidectomy is the most effective approach with excellent results in the management of hemorrhoid disease, but hemorrhoidectomy is not a simple procedure. one of the complications of this surgery is an injury to anal sphincters that can l...

Citation: Starčič Erjavec M, Predojević L and Žgur-Bertok D (2017) Commentary: Comparative Analysis of Phylogenetic Assignment of Human and Avian ExPEC and Fecal Commensal Escherichia coli Using the (Previous and Revised) Clermont Phylogenetic Typing Methods and its Impact on Avian Pathogenic Escherichia coli (APEC) Classification. Front. Microbiol. 8:1904. doi: 10.3389/fmicb.2017.01904 Comment...

background : microbial monitoring of aquatic sources is essential in the correct management of these vital sources. multiple tube fermentation (mtf) technique is currently used for assessment of the microbial quality of water sources. however, today enzymatic assay has been presented as an alternative approach for detecting indicator bacteria. the purpose of this study was to compare enzymatic ...