The high-affinity, type I Ig Fc receptor (Fc gamma RI) for human IgG1, human IgG3, murine IgG2a, and murine IgG3 is highly expressed on monocytes, neutrophils (PMN) in certain disease states, and phagocytes treated with interferon-gamma (IFN-gamma). We studied the activation of the human PMN oxidative burst and stimulated fluid pinocytosis induced by three monoclonal antibodies (MoAbs) directed...

We attempted to elicit active anaphylaxis to ovalbumin, or passive IgEor IgG 1 -dependent anaphylaxis, in mice lacking either the Fc e RI a chain or the FcR g chain common to Fc e RI and Fc g RI/III, or in mice lacking mast cells ( Kit W / Kit W-v mice), and compared the responses to those in the corresponding wild-type mice. We found that the FcR g chain is required for the death, as well as f...

An IgM mAb (BB10) was produced by immunization of mice with human eosinophils purified according to their abnormal low density ("hypodense" cells), and previously shown to exhibit increased IgE-dependent antiparasite cytotoxicity. This BB10 antibody, selected for positive fluorescence staining of hypodense blood or lung eosinophils and low or negative staining of normodense eosinophils or neutr...

Using three different Fc g receptor (Fc g R)-deficient mouse strains, we examined the induction of autoimmune hemolytic anemia by each of the four immunoglobulin (Ig)G isotype-switch variants of a 4C8 IgM antierythrocyte autoantibody and its relation to the contributions of the two Fc g R, Fc g RI, and Fc g RIII, operative in the phagocytosis of opsonized particles. We found that the four IgG i...

Fc receptors play a pivotal role linking the cellular the mechanisms regulating receptor coupling to PLD in intact cells where the components are endogenously ex-and humoral arms of the immune system [1–3]. Our previous studies have shown that the human high-pressed and functional. First, the presence of specific PLD isozymes in the cell membrane fraction was exam-affinity immunoglobulin G rece...

BACKGROUND The Pictorial Representation of Illness and Self Measure (PRISM) has been introduced as a visual measure of suffering. We explored the validity of a revised version, the PRISM-RII, in diabetes patients as part of the annual review. METHODS Participants were 308 adult outpatients with either type 1 or type 2 diabetes. MEASURES (1) the PRISM-RII, yielding Self-Illness Separation (S...

Brief ischemic episodes confer marked protection against myocardial stunning 1-3 d later (late preconditioning [PC] against stunning). The mechanism of this powerful protective effect is poorly understood. Although protein kinase C (PKC) has been implicated in PC against infarction, it is unknown whether it triggers late PC against stunning. In addition, the entire PKC hypothesis of ischemic PC...

To explore an issue of a human-like motion, we have constructed a human-like biped robot called WABIAN-RII (WAseda BIped humANoid robot-Revised II) that has a total of fortythree mechanical degrees of freedom (DOF); two six DOF legs, two ten DOF arms, a four DOF neck, four DOF in the eyes and a torso with a three DOF waist. For human-robot symbiosis, the biped humanoid robot is required to have...

BACKGROUND To determine the interaction effect between APOE polymorphism and lipid concentrations and alcohol use on spontaneous deep intracerebral hemorrhage (SDICH) risks. METHODS We enrolled 217 SDICH patients and 280 controls. Anthropometrics, personal history, and concentrations of total cholesterol (TC), high-density lipoprotein cholesterol, low-density lipoprotein cholesterol (LDL-c), ...

A protected 2-aminopurine nucleoside suitable for incorporation into oligodeoxynucleotides using phosphite triester chemical synthesis procedures has been prepared via oxidation of a purine hydrazino derivative with silver (I) oxide. Five oligodeoxynucleotides containing Eco RI and Bam HI recognition sites have been prepared such that, in the double stranded form, the 2-aminopurine base has eit...