Department of Cosmetic Science, Chia-Nan University of Pharmacy and Science, 60, Erh-Jen RD., Sec. 1, Jen-Te, Tainan (Zip code 717), Taiwan Taoyuan General Hospital, 1492, Chung-Shan Road, Taoyuan City, Taoyuan (zip code 330), Taiwan *Corresponding Author Email: [email protected] Phone: 886-6-266-4911 ext 2400 Fax: 886-6-266-7324 In present study, B16-F10 cell line was grown in 96-well ...

The extent of maturation of the oligosaccharide subunits of tumor cell glycoproteins appears to correlate with malignant potential, suggesting that modification of oligosaccharide structures may alter metastatic capacity. Castanospermine, a recently discovered inhibitor of glucosidase I, was tested for its effect on experimental metastasis of B16-F10 murine melanoma cells and was compared to tr...

UNLABELLED Beta-lapachone (beta-Lp) derived from the Lapacho tree is a potentially novel anticancer agent currently under clinical trials. Previous studies suggested that redox activation of beta-Lp catalyzed by NAD(P)H quinone oxidoreductase 1 (NQO1) accounted for its killing of cancer cells. However, the exact mechanisms of this effect remain largely unknown. Using chemiluminescence and ele...

Aims: Folate receptor (FR) is overexpressed in most cancer cells and activated macrophages entailed rheumatoid arthritis (RA). The aim of the current study was fabrication FR-targeted nanocarrier loaded with methotrexate (MTX) to magnify its therapeutic efficacy limit toxicity.
 Methodology: Folic acid-chitosan (FA-CS) conjugate synthesized characterized. MTX poly(d,l-lactide-co-glycolide)...

Anisaldehyde (4-methoxybenzaldehyde), previously reported as a tyrosinase inhibitor, did not inhibit melanogenesis in cultured B16-F10 melanoma cells but rather enhanced it. This adverse effect of anisaldehyde was accompanied by melanocytotoxicity in a dose-dependent manner up to 2 mM. The melanin content per cell at 1 mM was increased 5-fold compared to control and morphological observations s...

Salicylic acid slightly inhibited the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase noncompetitively without being oxidized. In contrast, 4-hydroxybenzoic acid did not inhibit this enzymatic oxidation if a longer reaction time was observed, although it suppressed the initial rate of the oxidation to a certain extent. Neither acid showed noticeable effects o...

Equine embryos were recovered nonsurgically 6.5 d after ovulation (Exp. 1) and those >200 tim were stored in one of three media: 1) Ham's F10 + 10% fetal calf serum (FCS) under 5% CO 2 , 5% O~and 90% No at 24 C (Ham's F10); 2) Minimal Essential Medium with Hank's balanced salts + 10% FCS in air (MEM) at 24 C or 3) MEM at 5 C n=lO/treatment). Embryos 4200/~m (n=10) were bisected microsurgically;...