designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. this construction should have all necessary components of a real gene. a good designed fusion gene construction could be cloned into a good and suitable host. clostridium perfringens is an important pathogen of humans and livestock and produce...

Alzheimer's disease (AD) is a multifactorial resulting from both genetic and environmental factors, which are pathologically defined by the accumulation of intracellular hyperphosphorylated tau protein, neurofibrils tangles, extracellular amyloid β protein in brain. The purpose this study to estimate potentially damaging effects missense single nucleotide polymorphisms (SNPs) BID, MAPK10 AGER g...

Fusion of two genes at DNA level produces a single protein, known as a chimeric protein. Immunotoxins are chimeric proteins composed of specific cell targeting and cell killing moieties. Bacterial or plant toxins are commonly used as the killing moieties of the chimeric immunotoxins. In this investigation, the catalytic domain of Shiga-like toxin (A1) was fused to human granulocyte macrophage ...

objective(s) leishmania can lead to a broad spectrum of diseases, collectively known as leishmaniasis. the a2 gene/ protein family could be one of the most eligible candidate factors of virulence in visceral leishmaniasis (vl). the previous results confirmed that in leishmania infantum, several a2 proteins are abundantly expressed by the amastigote, but not the promastigote stage. as there are ...

The Su(f) protein of Drosophila melanogaster shares extensive homologies with proteins from yeast (RNA14) and man (77 kD subunit of cleavage stimulation factor) that are required for 3' end processing of mRNA. These homologies suggest that su(f) is involved in mRNA 3' end formation and that some aspects of this process are conserved throughout eukaryotes. We have investigated the genetic and mo...

background: vascular calcification is an important stage in atherosclerosis. during this stage, vascular smooth muscle cells (vsmc) synthesize many osteogenic factors such as osteonectin (encoded by sparc). oxidative stress plays a critical role in atherosclerosis progression, and its accumulation in the vascular wall stimulates the development of atherosclerosis and vascular calcification. the...

Over the last decade an intensive research on the regulation o f gene expression in viral and anim al systems has led to the discovery o f m -ac ting regulatory sequences, the identification o f sequence-specific D N A -binding proteins (trans-acting factors), the characterization o f p ro ­ tein dom ains involved in D N A -pro tein recognition and binding as well as in protein-protein interact...

objective(s): adeno-associated virus type 2 (aav2) vectors are widely used for both experimental and clinical gene therapy. a recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. in this study, a fast and simple method is presented to generate aav2 vector helper plasmids encodin...

background :   vaculating cytotoxic a ( vaca ) gene is one of the multiple helicobacter pylori genotypes that produce a cytotoxin protein (vaca).this gene is a major cause of chronic peptic ulcers and gastric cancer. the aim of this study was to determine the correlation between vaca gene with peptic ulcer disease (pud) and non-ulcer dyspepsia (nud).   methods :    this was a case control study...