the aim of this study was production and partial purification of α-amylase enzyme by bacillus licheniformis. b. licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. optimal conditions for amylase production by b. licheniformis are incubation period of 120 h, temperature of 37 °c and ph 7.0. the α-amylase enzyme was purified by ion exchange chromatography ...

Stem cell (SC) and cancer stem cell (CSC) regulation is congregated around the dynamic role played by their niche that defines a domain-specific identity and functions. The major macromolecular components of any physiological niche are collectively referred to as Extra Cellular Matrix (ECM). Consequently, niche-determined governance of SC-CSC fate(s) is significantly wired by the ECM and its ch...

We present a cellular automaton model of clonal evolution in cancer aimed at investigating the emergence of the glycolytic phenotype. In the model each cell is equipped with a micro-environment response network that determines the behaviour or phenotype of the cell based on the local environment. The response network is modelled using a feed-forward neural network, which is subject to mutations...

Using an in vivo rat model of unilateral renal ischemia, we previously showed that the expression and distribution of fibronectin (FN), a major glycoprotein of plasma and the extracellular matrix, dramatically changes in response to ischemia-reperfusion. In the distal nephron in particular, FN accumulates in tubular lumens, where it may contribute to obstruction. In the present study, we examin...

The forces that arise from the actin cytoskeleton play a crucial role in determining the cell shape. These include protrusive forces due to actin polymerization and adhesion to the external matrix. We present here a theoretical model for the cellular shapes resulting from the feedback between the membrane shape and the forces acting on the membrane, mediated by curvature-sensitive membrane comp...

Advanced glycation end-products (AGEs) induce the production of reactive oxygen species (ROS) and extra cellular matrix (ECM) degradation via suppression neuropilin-1 (NRP-1) interaction with AGE-receptors (RAGE). This study aimed to reveal whether modulation NRP-1 by rosuvastatin (RT) prevents AGE-induced renal injury targeting RAGE/matrix metalloproteinase-2 (MMP-2) signaling in diabetic rats...

in order to achieve prolong corneal contact time of norfloxacin(nfx) for treatment of extra ocular diseases, a ph triggered nanoparticulate in situ gelling system was designed to explore dual advantage of nanoparticles and in situ gelling system, for its ocular delivery. nfx loaded nanocarriers were developed by ionotropic gelation technique using chitosan as a matrix forming polymer, cross-lin...

We report the case of a 59-year-old obese female who developed an abdominal wall haematoma during administration of prophylactic clexane. Compared with the non-obese, the subcutaneous tissue of the obese is considered dysfunctional and has a different vascular structure and extra-cellular matrix composition. While the development of an abdominal wall haematoma is relatively uncommon, when they ...

Cortactin, a substrate of sarcoma (Src) kinases, is an actin-binding protein that is involved in cytoskeletal regulation, and is frequently overexpressed in cancer cells. Binding to the actin related protein 2/3 (Arp2/3) complex stimulates cortactin activity, which promotes F-actin nucleation and assembly. Cortactin promotes cancer cell migration and invasion, and plays a pivotal role in invado...

Recently we have developed 4 channel incubation type planar patch clamp biosensor with polymethylmethacrylate (PMMA) substrate by using both-side hot embossing, focused ion beam micro-fabrication and surface modification by extra cellular matrix. We have evaluated the performance by using the light-gated ion-channel protein, channelrhodopsin wide receiver (ChRWR) expressed on HEK293 cell. Obser...