rna extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. although many rna extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. many studies have shown that trizol is an applicable material for the rna extracti...

human granulocyte colony stimulating factor (hg-csf) induces proliferation and differentiation of granulocyte progenitor cells. this glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. so far, different researchers have tried to enhance hg-csf biological activity and stability. in this study, polymerase chain reaction (p...

the genome of iranian wheat stripe virus (iwsv), a tentative member of the genus tenuivirus, is comprised of three ambisense and one negative sense rna segments. the coat and non-structural proteins encoded by the vcrna3 and vrna4 genes, respectively, were efficiently translated in vitro. translated proteins of vcrna3 and vrna4 transcripts were approximately 35000 and 22000 in mr, respectively,...

abstract: a pure nucleotide pool is an essential precursor of correct dna replication and prevention of mutagenesis and abnormality in a living cell. inosine triphosphatase (itpase) is a critical enzyme to remove any deaminated rough purine nucleotides such as inosine from nucleotide pool. it has been shown that the rate of substitution mutation in genomic dna increase by abnormal function and ...

background and objective: the association of ebv with hodgkin lymphoma (hl) has been intensely investigated over the last few years. ebv is also associated with several other malignancies. on the other hand, ki67 molecule serves as a widely accepted proliferation marker. several studies were previously performed about the expression of ki67 in hd. this study tried to detect the correlation betw...

streptococcus iniae is a major cause of serious bacterial infections in both fish and human beings. capsular polysaccharide (cps) of s. iniae is vital to evade phagocytic clearance of the host and serves as an important protective antigen of s. iniae infection in aquatic animals. the cpsdgene was determined to be highly conservative in capsule polysaccharide operon. prokaryotic expression of th...

plant fatty acyl-acyl carrier protein thioesterase (fat) is a major enzyme regulating the amount and composition of fatty acids in lipids. in this study, one type of cdna, corresponding to the fatty acyl-acyl carrier protein (acyl-acp) thioesterase (fat) enzyme, was isolated from the seed of brassica napus cv. ningyou12. blast results revealed that the cdna identified highly with the fatb class...

introduction: hepatitis c virus (hcv) is the major cause of acute and chronic hepatitis, cirrhosis, and hepatocellular carcinoma worldwide. because of increasing number of infected patients throughout the world, various studies are concentrated on development of high quality diagnostic tests and genotyping in various regions of the world. methods: in this study, we used rt-pcr to isolate and a...

objective: in this study, two conserved genes (m1 and np) of influenza virus were expressed in a bicistronic vector in order to develop a universal gene based vaccine. materials and methods: plasmids m1-pires2-egfp, pires2-np were constructed by cloning the pcr products of m1 and np genes which were amplified from the a/peurto rico/8/34 (h1n1) influenza virus strain into the plasmid expression...