BACKGROUND There is a need for new, noninvasive risk assessment tools for use in lung cancer population screening and prevention programs. METHODS To investigate the technical feasibility of determining DNA methylation in exhaled breath condensate, we applied our previously-developed method for tag-adapted bisulfite genomic DNA sequencing (tBGS) for mapping of DNA methylation, and adapted it ...

Cigarette smoking induces an inflammatory response in the airways that may play a key role in the pathogenesis of chronic obstructive pulmonary disease. Noninvasive markers of inflammation may, therefore, be useful in monitoring the airways of smokers as well as in the screening of subjects at high risk of developing airway obstruction. The aim of the present study was to determine whether the ...

BACKGROUND The early biological impact of short-term mechanical ventilation on healthy lungs is unknown. The authors aimed to characterize the immediate tidal volume (VT)-related changes on lung injury biomarkers in patients with healthy lungs and low risk of pulmonary complications. METHODS Twenty-eight healthy patients for knee replacement surgery were prospectively randomized to volume-con...

We would like to congratulate HUSZAR et al. [1] on their important and meticulous study demonstrating elevated adenosine levels in the exhaled breath condensate of atopic asthmatic subjects compared to nonatopic controls. Their findings are in agreement with and somewhat complementary to previous data obtained from bronchoalveolar lavage fluid of patients with asthma and chronic obstructive pul...

Spectrom Rev 2005; 24: 661–700. 6 Lee TW, Brownlee KG, Conway SP, et al. Evaluation of a new definition for chronic Pseudomonas aeruginosa infection in cystic fibrosis patients. J Cyst Fibros 2003; 2: 29–34. 7 Kumar S, Huang J, Abbassi-Ghadi N, et al. Selected ion flow tube mass spectrometry analysis of exhaled breath for volatile organic compound profiling of esophago-gastric cancer. Anal Chem...