Dormancy breaking and germination requirements were investigated in seeds of Ferula ovina and Ferula gummosa. Seeds of rather species were collected from north and eastern-north rangelands of Iran. Seeds of each of the species were subjected to different treatments including various levels of GA3, chilling and combination of GA3 and chilling (GA-chilling). In contrast to treated seeds, non-trea...

Establishment of efficient protocol for the induction of somatic embryogenesis and plantlet regeneration from shoot tip explants was achieved in Glycine max (L).Merr. cv.PK472. Highest frequency (95%) of embryogenic callus formation was obtained from shoot tip explants of Glycine max (L).Merr.cv.PK472 on Murashige and Skoog (MS) medium fortified with different concentration of 2,4-Dichloropheno...

Successful efficient whole cauliflower plant regeneration via somatic embryogenesis from root derived callus tissue was achieved. The research confirmed for the first time the capability of mass production of cauliflower somatic embryos through the indirect pathway. The best callus induction and proliferation was on semi solid Murashige and Skoog (MS) medium supplemented with 2, 4-D at 0.15 mg ...

Various plant organ segments including segments from roots (root tip, and root collar), stems, leaves, and also seeds of several Hyoscyamus species (i.e. H. pusillus, H. arachnoideus, annual and biennial H. niger from two distinct geographical regions) were cultured on MS media with various growth regulator combinations, to assess the ability of flower production. Statistical assessments showed...

introduction: the purpose of this study was to investigate the effect of rpmi and t6 medium on two cell mouse embryo co-culture with vero cells. materials and methods: two cell mouse embryos (nmri strain) were flushed from the excised uteri of superovulated mice. morphologically normal embryos were divided into four groups: the first control group was cultured on t6 media. the first experimenta...

Cumin (Cuminum cyminum L.) as a member of the Apiaceae family is one of the most important medicinal plants in Iran. The purpose of this study is to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. For this purpose the cumin seeds (Kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on MS basal medium. Leaf and hypo...

A prerequisite for most plant transformation systems is an efficient and reliable method to regenerate phenotypically normal plants. The present studies were undertaken with a view to the effects of Benzyl amino purine (BAP) and Indol butyric acid (IBA) hormones and genotypes on in vitro germination of immature embryos in walnut (Juglans regia L.). The immature embryos of walnut were excised fr...

BACKGROUND AND AIMS The formation of zonal communities may be attributed to differences in germination across the community and to timing of germination of seeds present in the seed bank. Our goals were two-fold: (1) to assess the annual germination pattern of Spergularia marina; and (2) to determine whether germination of S. marina differed across zonal communities. METHODS Fresh seeds were ...

An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...