Experimental leishmaniasis is widely used to study the effector functions of T helper cell subsets in vivo. Healing and nonhealing Leishmania major infections have been correlated with T helper 1 and T helper 2 responses, respectively. In the present study, we determined T cell effector functions ex vivo, without any further restimulation and compared them to those obtained following antigen-sp...

calcium hydroxide is the most widely used intracanal medicament in endodontics, which should be removed prior to permanent root canal filling to minimize its intervention with the bonding of endodontic sealers. this ex-vivo study aimed to evaluate the effect of pretreatment with calcium hydroxide on the bond strength of resilon/epiphany self etch (se) to the radicular dentin after removing the ...

objective: allogeneic transplantation with umbilical cord blood (ucb) in adult recipients is limited mainly by a low cd34+ cell dose. to overcome this shortcoming, human placenta as a novel source of human mesenchymal progenitor cell (mpc)- unrestricted somatic stem cells (ussc)- was incorporated in an attempt to expand cd34+ cells from ucb. to provide a similar environment in vitro, we coated ...

introduction: the purpose of this ex vivo study was to assess the effect of two root-end filling materials against candida (c) albicans . materials and methods: proroot mta and cem cement were compared immediately and 24 h after mixing, in two different concentrations (50 and 100 mg/ml). a total of 50 culture wells were used and divided into experimental (n=10) and control groups (n=5). those w...

AIM OF THE STUDY Resistance to imatinib is one of the most important issues in treatment of chronic myeloid leukemia (CML) patients. The objective of the study was to analyze the ex vivo drug resistance profile to bortezomib and 22 other antileukemic drugs, including three tyrosine kinase inhibitors (TKIs), in CML in comparison to acute myeloid leukemia (AML). MATERIAL AND METHODS A total of ...

Hypoxia is a characteristic feature of solid neoplasms, and insufficient oxygen supply increases cellular nicotinamide adenine dinucleotide (NADH) fluorescence, which is a main component of autofluorescence of the colorectal mucosa. We investigated whether a dual-wavelength excitation method which is optimized for sensing mucosal NADH fluorescence could be applicable to the detection of rat col...

PURPOSE We aimed to evaluate the validity of lung lobe weight assessment via computed tomography (CT) by comparing CT-derived and ex vivo measurements. MATERIALS AND METHODS Unenhanced CT scanning was performed in 30 consecutive patients before lobectomy for lung cancer. The CT images were analyzed using research software after allowing for lobar weight quantitation. The lobar weight estimate...

BACKGROUND To compare nondestructive in vivo and ex vivo micro-computed tomography (muCT) and ex vivo dual-energy-X-ray-absorptiometry (DXA) in characterizing mineralized cortical and trabecular bone response to prostate cancer involving the skeleton in a mouse model. METHODOLOGY/PRINCIPAL FINDINGS In vivo microCT was performed before and 10 weeks after implantation of human prostate cancer c...

BACKGROUND During peritoneal dialysis (PD), epithelial-mesenchymal transition (EMT) is likely involved in aberrant healing and progressive peritoneal fibrosis. Recently, EMT of the kidney was actively reversed into the opposite direction, into mesenchymal-epithelial transition (MET), by treatment with bone morphogenic protein-7 (BMP-7). In this study, the potential for ex vivo interconversion o...