Purified simian virus 40 has associated with it an endonuclease activity which converts form I (double-stranded, circular) simian virus 40 deoxyribonucleic acid to a nicked form that sediments as a homogeneous peak in alkaline sucrose gradients. The enzyme is dependent on magnesium ions for activity and is completely inhibited by ethylenediaminetetraacetic acid (0.02 m) or heat (80 C for 10 min...

An original functionalization strategy is proposed here to design chitosan (CS)-based cryogels with ethylenediaminetetraacetic acid (EDTA) moieties. Cryogels aligned micro-sized tubular structures were further engineered through an unidirectional freezing approach. Attachment of EDTA groups onto CS chains was proved by 1H-RMN and FT-IR spectroscopy. The formation EDTA-functionalized 3D porous C...

The C-8 position of deoxyguanosine (dGuo) was hydroxylated by ascorbic acid in the presence of oxygen (O2) in 0.1 M phosphate buffer (pH 6.8) at 37 degrees C. Addition of hydrogen peroxide (H2O2) remarkably enhanced this hydroxylation. The Udenfriend system [ascorbic acid, FeII, ethylenediaminetetraacetic acid (EDTA) and O2] was also effective for hydroxylation of dGuo in high yield. Guanine re...

Esters reduce to form ethers and alcohols on contact with metal nanoparticles supported Brønsted acidic faujasite (M-FAU) that cleave C−O bonds by hydrogenation hydrogenolysis pathways. Rates selectivities for each pathway depend the identity (M=Co, Ni, Cu, Ru, Rh, Pd, Pt). Pt-FAU gives propyl acetate consumption rates up 100 times greater than other M-FAU catalysts provides an ethyl ether sele...

Four new compounds have been synthesized as potential inhibitors of dihydroorotase from Escherichia coli. NMR spectroscopy was used to show that 4,6-dioxo-piperidine-2(S)-carboxylic acid (3), exists in solution as a mixture of the hydrate (7), enol (8), and enolate (9) tautomeric forms. This compound was found to be a competitive inhibitor versus dihydroorotate and thio-dihydroorotate at pH val...

The histological differentiation of neuronal from glial nuclei may be useful in the identification of tumours and in the study of developing nervous tissues. The following method does this by staining the glial nucleus red and the neuronal nucleus green. It also stains other structures in the neural parenchyma. Formol-fixed tissues taken down to water and embedded in paraffin are used. 1 The se...