A function for mitochondria in the reproduction of Rous sarcoma virus (RSV) in chronically and newly infected chick embryo cells was studied by using chloramphenicol and ethidium bromide. Chloramphenicol (CAM) and ethidium bromide (EB) were both shown to decrease the rate of growth of infected chick embryo cells and to inhibit the synthesis of mitochondrial macromolecules. Both drugs however ha...

Short pulse-labeling of log phase Crithidia fasciculata cells with [3H]thymidine allowed the autoradiographic visualization of 2 sites of replication of kinetoplast DNA situated at the periphery of the networks and separated by 180 degrees. Longer pulse-labeling led to the previously reported total peripheral labeling pattern. Pulse-labeled networks possess an intermediate density in ethidium b...

Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis. However, if these tools are to be used correctly and accurately, the techniques used must be standardised. In the present study, two molecular techniques for diagnosing Cryptosporidium infection in co...

Objective: Anticancer activities of Schiff bases S1 and S2 against MG-63 human osteosarcoma cells were performed described using an in vitro evaluation employing cytotoxicity apoptosis assay. Methods: used MTT assay to examine the effect compound on cell viability (S1 S2). Cell morphologies IC50 values obtained. The acridine orange (AO)/ethidium bromide (EB) dual staining technique was determin...

Techniques for preparing large populations of anucleate cytoplasms from cultured eukaryotic cells have only recently been described. The principal value of anucleate cytoplasms derives from studies that can be done after they are fused to whole cells. Since present methods for the isolation of heterokaryons are unsuitable for the selection of hybrids between whole cells and anucleate cytoplasms...