background: campylobacter jejune and c. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. in this study identification of campylobacter jejuni and campylobacter coli from samples using pcr was explored. methods: detection was performed using diarrheal samples collected 8 from 117 children. the genomic dna of samples was extracted by phenol-chloroform me...

background and objectives: the therapeutic options for diseases caused by escherichia coli are limited. in this study we investigated the presence of virulence factors among enteropathogenic escherichia coli (epec) strains and their antibiotic resistance patterns. the isolates were also checked for the presence of class1 integrons and gene cassettes. materials and methods: this study included 7...

background and objectives: shiga toxin-producing escherichia coli ( stec) have emerged as human pathogens and contamination of foods of animal origin has been a major public health concern. the aim of the present study was to determine the dissemination of stec in healthy and diarrheic calves in urmia region which is located in west azerbaijan province, iran. materials and methods: in the curre...

conclusions the analysis recommended that ta genes are prevalent in clinical isolates of e. coli strains. the analysis revealed that hipba ta system is associated with biofilm formation. results the results of the analysis revealed that 107 e. coli isolates were potent for biofilm formation by cra. the findings by microtiter plates showed that 102 e. coli isolates were biofilm producers. the re...

conclusions the alcoholic extract of p. harmala was very effective against the selected esbl-producing e. coli isolates harboring the tem gene. therefore, it could be suggested as an antibacterial agent in the future. more researches are necessary for detecting the mechanism of this plant’s behavior and its pharmacological effects. results results of this study showed that in disk diffusion met...

conclusions: our data showed that the recombinant mature lysostaphin protein produced by pet32a vector in e. coli system was very efficient. results: pcr and sequencing results confirmed the successful cloning of the target gene into the vector. the expression of protein was induced by iptg and high concentration of the recombinant protein was obtained via the purification process by affinity-c...

conclusions anova results showed that the effect of cycle and time on the selected bacterial removal efficiency were more important than that of the microbial concentration. results the results showed that e. coli and e. faecalis were effectively treated at 10 pulse/s in 9 minutes and 6 log cfu/ml bacterial suspension (p < 0.0001 for e. coli with 99.99% (4 log) and p = 0.0002 for e. faecalis wi...

background genetic manipulation of chloroplast in higher plants offers a number of unique prerogatives, including; undesirable of pleiotropic genome and gene silencing effects and also use as an important agronomic trait for producing essential biomaterials and industrial enzymes. in order to manipulate chloroplast genome, specific vectors are required. these vectors can be transformed and expr...

In this study, to know about the phylogeny of Escherichia coli isolated from broilers with collibacillosis in Tabriz, 70 E. coli isolates recovered from broilers with collibacillosis were characterized for phylogenetic group (A, B1, B2, D) by multiplex PCR. Of the all 70 samples, 35 (50%) isolates were classified as type A, 32 (45%) as type D, 2 (2.8%) as type B1 and 1 (2.8%) as type B2. This s...

data describing the microbiological status of a country’s meat products can be of great value: in acting as a de facto validation of the country’s regulatory systems, as a baseline for setting performance standards and in supporting risk assessment. o26, o45, o91, o103, o111, o113, o121, o128, o145, and o157 are the most common serotypes of enterohemorrhagic escherichia coli associated with hum...