background and objectives: β-lactamase production is the predominant mechanism for resistance to β-lactams in enterobacteriaceae. the producing isolates are often multidrug resistant and are major hosts for extended spectrum β-lactamases. this study was designed to determine the prevalence of extended-spectrum β-lactamase producing isolates of escherichia coli and klebsiella pneumoniae and thei...

We determined the faecal carriage of extended-spectrum beta-lactamase-(ESBL)-producing bacteria in the community in Saudi Arabia. A total of 716 faecal specimens (from 505 healthy individuals and 211 community outpatients) were screened for ESBL using the double-disk synergy test and confirmed by the Clinical Laboratory Standards Institute combined disk method. We found 91 (12.7%) isolates were...

Sepsis is an organ dysfunction caused by dysregulation of immune response towards systemic infection with high incidence and mortality rate. Innovative breakthrough required to manage sepsis especially extended spectrum beta lactamase (ESBL) producing Escherichia coli (E. coli) induced sepsis. Utilization Piper betle extract could be considered as alternative therapy should proven scientificall...

We rapidly identified extended-spectrum β-lactamase (ESBL) producers prospectively among 245 gram-negative bacilli-positive cultured blood specimens using the Rapid ESBL Nordmann/Dortet/Poirel test and direct bacterial identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. This combination identified ESBL-producing Enterobacteriaceae within 30 min and...

The aim of the study was to determine the genotype of 15 ESBL strains of Enterobacteriaceae resistant to beta-lactams, isolated from inanimate surfaces and phenotypically characterized as producing extended-spectrum beta-lactamase. After evaluation and screening of the bacterial strains, a PCR was conducted to amplify fragments of 1078 bp and 544 bp corresponding to type TEM and CTX-M ESBL. Ele...

ChromID ESBL agar and Brilliance ESBL agar were compared for the isolation of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae from 298 stools. These had comparable sensitivity and selectivity for the 116 positive samples. Pre-enrichment with cefpodoxime and extending incubation to 48 hours after direct plating both significantly increased sensitivity but reduced selectivity of...

background: beta-lactamase enzymes are considered the most important factor of resistance against b-lactam antibiotics among gram-negative bacteria. in recent years, the production of extended-spectrum b-lactamases has been prevailed among bacteria, especially bacteria of animal origin, and this is important in terms of public health. objective: the prupose of this study is to evaluate the pres...

Results of a 2003 survey carried out in Italy to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing enterobacteria are presented. Eleven Italian Microbiology Laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). ESBL screening was performed by MIC data analysis. Confirmation was obtained using the double-disk synergy...

This study was carried out to determine the prevalence of Extended-spectrum -lactamase (ESBL) and their antibiotic resistance patterns from clinical isolates in a university hospital. A total of One hundred and seventy-two isolates of Gram-negative were identified using Standard Laboratory Methods from various clinical specimens. Antibiotics susceptibility test was performed using disc diffusio...

A total of 113 blood culture isolates of Klebsiella pneumoniae from 10 hospitals in northern Taiwan were studied for SHV and TEM beta-lactamase production. bla(SHV) was amplified from all isolates by PCR. TEM-type resistance, was found in 32 of the isolates and was of the TEM-1 type in all isolates. SHV-1, -2, -5, -11, and -12 and two novel enzymes were identified. These novel enzymes were desi...