BACKGROUND The prion protein (PrP) might be useful as a tool to collect cardiac progenitor cells derived from embryonic stem (ES) cells. It is also possible that PrP(+) cells include undifferentiated cells with a capacity to develop into tumors. METHODS PrP(+) cells isolated from embryoid bodies (EB) formed by mouse AB1 ES cells were examined using RT-PCR analysis and clonogeneic cell assay. ...

CD99 is a 32kDa surface glycoprotein that is involved in the migration of leukocytes, cell-cell adhesion and apoptosis of T cells and Ewing's sarcoma (ES) cells, two cell types with a high level of CD99 expression. Engagement of the molecule induces a rapid death signal that appears to be related to the level of expression of this antigen. The rapid apoptosis induced by agonistic anti-CD99 mono...

The following analytes were determined with the automated enzyme immunoassay unit, Enzymun-Test System ES 300, and by routine in-house tests, and the results were compared: thyrotropin, free thyroxine, cortisol, immunoglobulin E, digoxin, digitoxin, insulin and carcinoembryonic antigen. The methods used for comparison included two radioimmunoassays, one with enzyme labelling, two with fluoresce...

The endolysosomal system (ES) consists of lysosomes; early, late, and recycling endosomes; and autophagosomes. It is a key regulator not only of macromolecule degradation and recycling, plasma membrane repair, homeostasis, and lipid storage, but also of antigen presentation, immune defense, cell motility, cell death signaling, tumor growth, and cancer progression. In addition, it plays a critic...

Among advanced melanomas, approximately 60% of the patients present with immunologic ignorance or intrinsic induction tumors need other therapeutic approaches to change an immunologically “cold tumor” “hot by attracting T cells into tumors. We have established immune cell therapy immortalized induced pluripotent stem–cell (iPSC)–derived proliferating myeloid (iPSC–pMCs). The benefits using iPSC...

The way to overcome current limitations in the generation of glucose-responsive insulin-producing cells is selective enrichment of the number of definitive endoderm (DE) progenitor cells. Sox17 is the marker of mesendoderm and definitive endoderm. The aim of the present research was to study the potential of Sox17 knock-in CGR8 mouse embryonic stem (ES) cells to differentiate into insulin produ...

Purpose: The present study aimed to investigate the effect of voluntary and electrically stimulated quadriceps muscle fatigue on postural control. Methods: Sixteen healthy young males participated in this quasi-experimental study. All subjects performed two fatiguing exercises with equal intensity of isometric maximal voluntary contraction: Voluntary (VOL) and Electrical Stimulation (ES). The ...