A decision-making protocol/tool is developed in which bark analytical data can be the input to predict most appropriate enzymic systems employ.

1. The influence of temperature, moisture content and the presence of glucose on the level of available methionine and cysteine in casein was studied. 2. Differences between total and available methionine and cysteine contents of heated casein (90 degrees for 24 h) were determined by an in vitro method. The maximum losses in total and available methionine content were 22 and 51% respectively. T...

Cytidine 5'-triphosphate synthase catalyses the ATP-dependent formation of CTP from UTP with either ammonia or glutamine as the source of nitrogen. When glutamine is the substrate, GTP is required as an allosteric effector to promote catalysis. Escherichia coli CTP synthase, overexpressed as a hexahistidine-tagged form, was purified to high specific activity with the use of metal-ion-affinity c...