A new lipolytic strain of Bacillus sp. was isolated from oil contaminated soil sample. On measurement of biochemical parameters and 16S rDNA sequence information the strain was identified as Bacillus pumilus RK31 (NCBI accession no GQ463238). The lipase purification steps involved, 60% ammonium sulphate saturation, gel filtration chromatography using Sephadex G200 and ion exchange chromatograph...

A purification procedure for the preparation of chicken liver arginase in a homogeneous form is presented. The enzyme hydrolyses both arginine and argininic acid. Kinetic analysis reveals that the enzyme binds arginine when the amino group is protonated or unprotonated; however, the unprotonated form seems to be hydrolysed more rapidly. The enzyme exchanges with the medium approx. 1.6Mn2+ ions ...

In this study, the cloning, purification and initial characterization of carbonic anhydrase (DrCA) enzyme which we consider to be important in resistance physiology from extremely radioresistant bacteria Deinococcus radiodurans is performed. addition, effect increased gamma irradiation doses on pH-related DrCA activity was determined. after radiation treatment showed that continuously by 6 fold...

A procedure for the purification of the enzyme bile acid:CoA ligase from guinea pig liver microsomes was developed. Activity toward chenodeoxycholate, cholate, deoxycholate, and lithocholate co-purified suggesting that a single enzyme form catalyzes the activation of all four bile acids. Activity toward lithocholate could not be accurately assayed during the earlier stages of purification due t...

Plasminogen (profibrinolysin) is the precursor of a proteolytic enzyme, plasmin (fibrinolysin), which is found in the euglobulin fraction of blood and which actively lyses clots in vitro and in vivo. The most satisfactory procedures for the purification of this enzyme precursor have been reported by Remmert and Cohen (1) and Christensen and Smith (2). The former investigators, by means of repea...

(2000) Purification of recombinant hydantoinase and L-N-carbamoylase from Arthrobacter aurescens expressed in E. coli: Comparison of wild-type and genetically modified proteins. J. Chromatogr. B. Abstract Two enzymes, hydantoinase (HyuH) and L-N-carbamoylase (HyuC), are required for the biocatalytic production of natural and unnatural, optically pure L-amino acids starting from D,L-5-monosubsti...

fermentation is an important process for black tea production. during this process, by exposure of substrate to enzymes and the underlying oxidation reactions, quality determining factors of tea is produced. it is known that the exogenous application of cell-wall degrading enzymes leads to the increased production of these compounds through enhancement of enzyme-substrate interactions. pectinas...

Objective: L-asparaginase has been isolated from a wide variety of microbial as well as plant and animal sources. In the study the production, partial purification and kinetic characterization of L-asparaginase from Pseudomonas fluorescens were carried. Methods: L-asparaginase was produced by submerged fermentation method. The purification of L-asparaginase was done through ammonium sulphate pr...

The purification of thermo-acidic amylase enzyme from red pitaya (Hylocereus polyrhizus) peel for the first time was investigated using a novel aqueous two-phase system (ATPS) consisting of a thermo-separating copolymer and an organic solvent. The effectiveness of different parameters such as molecular weight of the thermo-separating ethylene oxide-propylene oxide (EOPO) copolymer and type and ...

BACKGROUND Cre recombinase is a common reagent used for the in vivo on/off switching of the expression of target genes flanked by loxP sites. In particular, recombinant TAT-Cre fusion constructs purified from bacteria have been used to promote the cell uptake of the enzyme. However, the recovery of active TAT-Cre remains a demanding process and its specific activity varies significantly among b...