Production of intracellular β-xylosidase was studied in cultures of Penicillium janthinellum grown on citrus fruit waste supplemented cultivation media. Both dried orange peel and sweet lime peel could induce the production of this enzyme. The working strain showed a pronounced optimum pH and temperature for β-xylosidase production at 6.0 and 27 °C, respectively. The enzyme production was found...

Doxorubicin (DOX) is one of the drugs necessary for treatment 10 most common types cancer. The leading adverse effect limiting clinical use DOX cardiotoxicity. Given that literature data indicate a protective role carotenoids in doxorubicin-induced toxicity, our study we compared cardioprotective mixture pumpkin and commercially available antioxidant preparation. Animals were distributed 8 grou...

Essential oils extracted from plants contain protective volatile compounds and are known to processes antibacterial, antifungal, anti-oxidative, anti-inflammatory effects. This study was conducted explore the immunomodulatory effects of essential oil Chamaecyparis obtusa (EOCO) on house dust mite-induced mucosal inflammation. Cultured primary nasal epithelial cells were stimulated with Dermatop...

Partial recovery of ultraviolet-damaged denatured or native transforming DNA from Hemophilus influenzae, has been obtained by exposing the irradiated DNA in the denatured form to nitrous acid. Some factors that affect this recovery are described. An erythromycin marker (E(20)) was not reactivated. The UV damage reactivable by nitrous acid is different from that repaired by the photoreactivating...

The expansion of the dry grind ethanol industry provides a unique opportunity to introduce cellulose conversion technology to existing grain to ethanol plants, while enhancing ethanol yields by up to 14%, and decreasing the volume while increasing protein content of distiller's grains. The technologies required are cellulose pretreatment, enzyme hydrolysis, fermentation, and drying. Laboratory ...

Using various Sodium hydroxide (NaOH) concentrations (0, 1, 2, and 3%), we studied the alkaline pretreatment of microalgal biomass from blue-green alga Nostochopsis lobatus in this work. In stage, entrapped polysaccharides found within microalgae cell walls are broken down to form fermentable subunits. The selected procedure (2% NaOH) was then followed by enzymatic saccharification for generati...

A critical parameter affecting the economic feasibility of lignocellulosic bioconversion is the production of inexpensive and highly active cellulase enzymes in bulk quantity. A promising approach to reduce enzyme costs is to genetically transform plants with the genes of these enzymes, thereby producing the desired cellulases in the plants themselves. Extraction and recovery of active proteins...

In the present study, we assessed the role of tissue angiotensin-converting enzyme as a determinant of intrarenal hemodynamics by using the angiotensin-converting enzyme inhibitor trandolaprilat and the angiotensin II receptor antagonist losartan. Afferent and efferent arteriolar diameters were measured with computer-assisted vessel imaging in isolated perfused hydronephrotic rat kidneys. In re...

Abstract Corn (Zea mays) is a commodity of food crops that are important after rice in Indonesia. plantation produces waste the form corncob. Corncob lignocellulosic biomass can converted to cellulose, even sugar or another chemical like ethanol. The first step for utilization corncob delignification. purpose this study was isolate and purify enzyme laccase from white rot fungus Cerrena sp.B.Md...

Here, we describe the development of a bi-enzymatic biosensor that simplifies the sample pretreatment steps for insecticide detection, and opens the way for a highly sensitive detection of phosphorothionates in food. These compounds evolve their inhibitory activity towards acetylcholinesterases (AChEs) only after oxidation, which is performed in vivo by P450 monooxygenases. Consequently, phosph...