This study examines the prolactin hormone profile in Etaawa, Sapera and Saanen lactating goats, adult females, pregnant does, yearlings. used a total of 80 randomly selected goats with details 26 Ettawa Crossbreed (eight lactating, six pregnant, heifers), 32 twelve yearlings) 22 (five four yearling). All were declared clinically healthy. Blood sampling was performed through jugular vein each an...

An enzyme-linked immunosorbent assay (ELISA) was developed to detect bovine antibodies to Histophilus somni exopolysaccharide (EPS), which is created during biofilm formation. When an index value of 0.268 was used, the sensitivity of the assay for infected calves was 90.5% at 3 weeks postinfection, but the number of positive animals increased by week 4. The specificity of the assay for healthy ...

This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-...

The results of meconium specimens and fortified samples screened for drugs of abuse by both enzyme-linked immunosorbent assay (ELISA, Immunalysis) and biochip microarray (Randox) methods were compared. The ELISA method was semi-automated using a TECAN Genesis. The Randox assay used the Randox Evidence Investigator system. Previously validated gas chromatography-mass spectrometry (GC-MS), GC-GC-...

fast enzyme linked immunosorbent assay (fast-elisa) was compared with the standard elisa for the diagnosis of human hydatidosis. seventy serum samples including 30 from hydatidosis patients (surgically confirmed), healthy control individuals not infected with any parasitic diseases (n=/20) and from others with different parasitic infections including, toxocariosis (n=5), fasciolosis (n=5), tric...

To develop the large scale serological assay for severe fever with thrombocytopenia syndrome virus (SFTSV) infection, we evaluated two different enzyme-linked immunosorbent assay (ELISA) methods using nucleocapsid protein (NP) and Gn proteins of CB1 (genotype B) SFTSV strains. The NP-based ELISA tests showed more sensitive with broad cross-reactivity between two different genotype A and B strai...

Sığır yetiştiriciliğinde önemli sorunlardan biri olan yeni doğan buzağı ishallerinde sığır Coronavirus (BCoV) enfeksiyonları çok sık görülmektedir. Bu çalışmanın amacı Şanlıurfa ilindeki ishalli buzağılarda BCoV enfeksiyonunun araştırılmasıdır. çalışmada, klinik muayeneyi takiben (≤3 ay) 94 örneklenmiştir. antijen varlığının tespiti için Enzyme-linked immunosorbent assay (ELISA) kullanıldı. Top...